32548

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3752501..3753042 Replicon chromosome
Accession NZ_AP019189
Organism Escherichia coli strain M217

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag B7R44_RS18850 Protein ID WP_000615983.1
Coordinates 3752764..3753042 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag B7R44_RS18845 Protein ID WP_000729703.1
Coordinates 3752501..3752761 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7R44_RS18825 3748176..3748961 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
B7R44_RS18830 3748933..3750645 + 1713 Protein_3515 flagellar biosynthesis protein FlhA -
B7R44_RS18835 3750869..3751366 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
B7R44_RS18840 3751542..3752291 - 750 WP_000056849.1 C40 family peptidase -
B7R44_RS18845 3752501..3752761 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B7R44_RS18850 3752764..3753042 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B7R44_RS18855 3753198..3753938 + 741 WP_001225679.1 murein L,D-transpeptidase -
B7R44_RS18860 3753909..3754676 - 768 WP_000333380.1 class II glutamine amidotransferase -
B7R44_RS18865 3754882..3755460 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3746304..3761584 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T32548 WP_000615983.1 NZ_AP019189:3752764-3753042 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T32548 NZ_AP019189:3752764-3753042 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT32548 WP_000729703.1 NZ_AP019189:3752501-3752761 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT32548 NZ_AP019189:3752501-3752761 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References