Detailed information of TA system TA032547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3746304..3746998
• Replicon: chromosome
• Accession: NZ_AP019189
• Organism: Escherichia coli strain M217

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: B7R44_RS18810
• Protein ID: WP_001263489.1
• Coordinates: 3746304..3746702 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: B7R44_RS18815
• Protein ID: WP_000554758.1
• Coordinates: 3746705..3746998 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3741892..3741972	-	81	NuclAT_11	-	-
-	3741892..3741972	-	81	NuclAT_11	-	-
-	3741892..3741972	-	81	NuclAT_11	-	-
-	3741892..3741972	-	81	NuclAT_11	-	-
B7R44_RS18785	3742568..3743026	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
B7R44_RS18790	3743287..3744744	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
B7R44_RS18795	3744801..3745322	-	522	Protein_3508	peptide chain release factor H	-
B7R44_RS18800	3745318..3745524	-	207	Protein_3509	RtcB family protein	-
B7R44_RS18805	3745842..3746294	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
B7R44_RS18810	3746304..3746702	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
B7R44_RS18815	3746705..3746998	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
B7R44_RS18820	3747050..3748105	-	1056	WP_001226164.1	DNA polymerase IV	-
B7R44_RS18825	3748176..3748961	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
B7R44_RS18830	3748933..3750645	+	1713	Protein_3515	flagellar biosynthesis protein FlhA	-
B7R44_RS18835	3750869..3751366	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3746304..3761584	15280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T32547 WP_001263489.1 NZ_AP019189:c3746702-3746304 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T32547 NZ_AP019189:c3746702-3746304 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT32547 WP_000554758.1 NZ_AP019189:c3746998-3746705 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT32547 NZ_AP019189:c3746998-3746705 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3