32533

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1856229..1856731 Replicon chromosome
Accession NZ_AP019189
Organism Escherichia coli strain M217

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B7R44_RS09490 Protein ID WP_000767829.1
Coordinates 1856477..1856731 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B7R44_RS09485 Protein ID WP_001259255.1
Coordinates 1856229..1856480 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7R44_RS09460 1851406..1852473 - 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
B7R44_RS09465 1852473..1853543 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
B7R44_RS09470 1853540..1854844 - 1305 WP_001446927.1 histidinol dehydrogenase -
B7R44_RS09475 1854850..1855749 - 900 WP_032329340.1 ATP phosphoribosyltransferase -
B7R44_RS09480 1855895..1855945 - 51 WP_001364200.1 his operon leader peptide -
B7R44_RS09485 1856229..1856480 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B7R44_RS09490 1856477..1856731 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B7R44_RS09495 1856814..1857638 + 825 WP_000754749.1 SDR family oxidoreductase -
B7R44_RS09500 1857684..1858613 + 930 WP_000803351.1 LysR family transcriptional regulator -
B7R44_RS09505 1858828..1858890 + 63 WP_010723108.1 membrane protein YoeI -
B7R44_RS09510 1858880..1860238 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B7R44_RS09515 1860417..1861475 + 1059 WP_000492321.1 transport protein YeeE -
B7R44_RS09520 1861489..1861716 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32533 WP_000767829.1 NZ_AP019189:1856477-1856731 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32533 NZ_AP019189:1856477-1856731 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32533 WP_001259255.1 NZ_AP019189:1856229-1856480 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32533 NZ_AP019189:1856229-1856480 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References