Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1856229..1856731 Replicon chromosome
Accession NZ_AP019189
Organism Escherichia coli strain M217

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B7R44_RS09490 Protein ID WP_000767829.1
Coordinates 1856477..1856731 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B7R44_RS09485 Protein ID WP_001259255.1
Coordinates 1856229..1856480 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7R44_RS09460 1851406..1852473 - 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
B7R44_RS09465 1852473..1853543 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
B7R44_RS09470 1853540..1854844 - 1305 WP_001446927.1 histidinol dehydrogenase -
B7R44_RS09475 1854850..1855749 - 900 WP_032329340.1 ATP phosphoribosyltransferase -
B7R44_RS09480 1855895..1855945 - 51 WP_001364200.1 his operon leader peptide -
B7R44_RS09485 1856229..1856480 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B7R44_RS09490 1856477..1856731 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B7R44_RS09495 1856814..1857638 + 825 WP_000754749.1 SDR family oxidoreductase -
B7R44_RS09500 1857684..1858613 + 930 WP_000803351.1 LysR family transcriptional regulator -
B7R44_RS09505 1858828..1858890 + 63 WP_010723108.1 membrane protein YoeI -
B7R44_RS09510 1858880..1860238 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B7R44_RS09515 1860417..1861475 + 1059 WP_000492321.1 transport protein YeeE -
B7R44_RS09520 1861489..1861716 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32533 WP_000767829.1 NZ_AP019189:1856477-1856731 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32533 NZ_AP019189:1856477-1856731 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32533 WP_001259255.1 NZ_AP019189:1856229-1856480 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32533 NZ_AP019189:1856229-1856480 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References