Detailed information of TA system TA032488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2419125..2419855
• Replicon: chromosome
• Accession: NZ_AP019007
• Organism: Enterobacter oligotrophicus strain CCA6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EoCCA6_RS11650
• Protein ID: WP_152082789.1
• Coordinates: 2419541..2419855 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EoCCA6_RS11645
• Protein ID: WP_152082788.1
• Coordinates: 2419125..2419544 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EoCCA6_RS11620	2414619..2416124	-	1506	WP_152082785.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
EoCCA6_RS11625	2416121..2416714	-	594	WP_152082786.1	transcriptional regulator UhpA	-
EoCCA6_RS11630	2416797..2417084	-	288	WP_010426528.1	acetolactate synthase small subunit	-
EoCCA6_RS11635	2417088..2418776	-	1689	WP_152082787.1	acetolactate synthase large subunit	-
EoCCA6_RS11640	2418879..2418977	-	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
EoCCA6_RS11645	2419125..2419544	-	420	WP_152082788.1	helix-turn-helix domain-containing protein	Antitoxin
EoCCA6_RS11650	2419541..2419855	-	315	WP_152082789.1	type II toxin-antitoxin system HigB family toxin	Toxin
EoCCA6_RS11655	2420344..2420433	+	90	WP_071599731.1	type I toxin-antitoxin system toxin TisB	-
EoCCA6_RS11660	2420498..2420944	+	447	WP_152082790.1	GNAT family N-acetyltransferase	-
EoCCA6_RS11665	2421007..2421840	+	834	WP_152082791.1	DMT family transporter	-
EoCCA6_RS11670	2422016..2423200	+	1185	WP_152082792.1	multidrug efflux MFS transporter EmrD	-
EoCCA6_RS11675	2423103..2424191	-	1089	WP_152082793.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12401.52 Da
• Isoelectric Point: 10.2509

>T32488 WP_152082789.1 NZ_AP019007:c2419855-2419541 [Enterobacter oligotrophicus]
MHLISMKAILDAVTQFPQHKEELLFLGRIIEKSHCPTPAALRKIFPTLDNFKYLDKHYVIDVANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T32488 NZ_AP019007:c2419855-2419541 [Enterobacter oligotrophicus]
ATGCATTTAATCTCTATGAAGGCAATTTTGGATGCGGTAACCCAGTTTCCGCAGCACAAGGAAGAACTTCTTTTTTTAGG
ACGTATTATCGAAAAAAGCCATTGCCCAACGCCTGCTGCGCTAAGAAAAATCTTTCCGACGCTAGATAACTTCAAATATC
TCGATAAGCATTATGTTATTGATGTTGCAAACAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15479.07 Da
• Isoelectric Point: 5.2068

>AT32488 WP_152082788.1 NZ_AP019007:c2419544-2419125 [Enterobacter oligotrophicus]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLINEPGSPLLDLVCARISRYEANRPEMVALRQEMESVPVG
IAVLRTLMDQYKLTLSDFQDEIGSKSMVSRVLNGQRQLTLSHIKKLAARFGISPALFIE

Nucleotide

• Length: 420 bp

>AT32488 NZ_AP019007:c2419544-2419125 [Enterobacter oligotrophicus]
ATGATGATCGTCGCTGATGCAATGAAAGCAACGCATGCCCTGGTTGCCGCTGTTCCTTTACTGGGTGAACATCCGAACGA
GAAAGATTACCAGGACGCACTCGAACTGGTGGAATATTTGCTCATAAACGAGCCTGGTAGCCCGTTACTGGATCTGGTGT
GCGCCAGAATTAGCCGTTACGAAGCGAACCGACCTGAAATGGTCGCATTACGCCAGGAGATGGAATCTGTTCCAGTAGGG
ATTGCGGTTTTAAGAACCCTTATGGATCAATACAAACTGACTCTCTCCGATTTTCAGGATGAAATCGGCAGTAAATCAAT
GGTTTCAAGGGTGCTTAATGGGCAGCGACAGCTCACGTTGAGCCACATTAAAAAACTGGCGGCCCGCTTTGGTATATCGC
CTGCGCTTTTTATTGAGTGA