Detailed information of TA system TA032394

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 5602507..5602919
• Replicon: chromosome
• Accession: NZ_AP018808
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0F3WCK2
• Locus tag: EL389_RS29200
• Protein ID: WP_000132621.1
• Coordinates: 5602507..5602848 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	5602843..5602919 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS29185	5598517..5599929	-	1413	WP_000199352.1	PLP-dependent aminotransferase family protein	-
EL389_RS29190	5600106..5600270	+	165	WP_000394276.1	DUF1127 domain-containing protein	-
EL389_RS29195	5600367..5602460	+	2094	WP_000648228.1	DUF262 domain-containing protein	-
EL389_RS29200	5602507..5602848	-	342	WP_000132621.1	endoribonuclease SymE	Toxin
-	5602843..5602919	+	77	NuclAT_28	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_28	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_28	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_28	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_29	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_29	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_29	-	Antitoxin
-	5602843..5602919	+	77	NuclAT_29	-	Antitoxin
EL389_RS29205	5603069..5604838	-	1770	WP_000110079.1	restriction endonuclease subunit S	-
EL389_RS29210	5604838..5606307	-	1470	WP_001341289.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12342.12 Da
• Isoelectric Point: 7.8218

>T32394 WP_000132621.1 NZ_AP018808:c5602848-5602507 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T32394 NZ_AP018808:c5602848-5602507 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCACCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTAATTAC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT32394 NZ_AP018808:5602843-5602919 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0F3WCK2

Antitoxin