Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2787248..2787750 Replicon chromosome
Accession NZ_AP018808
Organism Escherichia coli strain E2865

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EL389_RS15040 Protein ID WP_000767829.1
Coordinates 2787248..2787502 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EL389_RS15045 Protein ID WP_001259255.1
Coordinates 2787499..2787750 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL389_RS15010 2782264..2782491 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EL389_RS15015 2782505..2783563 - 1059 WP_024241609.1 transport protein YeeE -
EL389_RS15020 2783742..2785100 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EL389_RS15025 2785090..2785152 - 63 WP_010723108.1 membrane protein YoeI -
EL389_RS15030 2785366..2786295 - 930 WP_000803351.1 LysR family transcriptional regulator -
EL389_RS15035 2786341..2787165 - 825 WP_000754737.1 SDR family oxidoreductase -
EL389_RS15040 2787248..2787502 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EL389_RS15045 2787499..2787750 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EL389_RS15050 2788033..2788080 + 48 WP_001375657.1 his operon leader peptide -
EL389_RS15055 2788226..2789125 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EL389_RS15060 2789131..2790435 + 1305 WP_000009589.1 histidinol dehydrogenase -
EL389_RS15065 2790432..2791502 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
EL389_RS15070 2791502..2792569 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32384 WP_000767829.1 NZ_AP018808:c2787502-2787248 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32384 NZ_AP018808:c2787502-2787248 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32384 WP_001259255.1 NZ_AP018808:c2787750-2787499 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32384 NZ_AP018808:c2787750-2787499 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References