Detailed information of TA system TA032367

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1870067..1870438
• Replicon: chromosome
• Accession: NZ_AP018808
• Organism: Escherichia coli strain E2865

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EL389_RS10070
• Protein ID: WP_001443846.1
• Coordinates: 1870289..1870438 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1870067..1870245 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL389_RS10045	1866022..1867395	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EL389_RS10050	1867524..1868459	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EL389_RS10055	1868511..1869746	-	1236	WP_000040851.1	site-specific integrase	-
EL389_RS10060	1869748..1869963	-	216	WP_000079604.1	excisionase XisR	-
EL389_RS10065	1870063..1870251	-	189	WP_001302840.1	DUF1187 family protein	-
-	1870067..1870245	+	179	NuclAT_0	-	Antitoxin
-	1870067..1870245	+	179	NuclAT_0	-	Antitoxin
-	1870067..1870245	+	179	NuclAT_0	-	Antitoxin
-	1870067..1870245	+	179	NuclAT_0	-	Antitoxin
EL389_RS10070	1870289..1870438	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EL389_RS10075	1870494..1871303	-	810	WP_000166313.1	recombination protein RecT	-
EL389_RS10080	1871296..1873896	-	2601	WP_000105151.1	exodeoxyribonuclease VIII	-
EL389_RS10085	1873998..1874273	-	276	WP_000632297.1	protein RacC	-
EL389_RS10090	1874348..1874518	-	171	WP_001352098.1	conserved protein, Rac prophage	-
EL389_RS10095	1874518..1874739	-	222	WP_000560223.1	killing protein KilR	-
EL389_RS10100	1875160..1875312	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1861252..1917065	55813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T32367 WP_001443846.1 NZ_AP018808:c1870438-1870289 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T32367 NZ_AP018808:c1870438-1870289 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT32367 NZ_AP018808:1870067-1870245 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin