Detailed information of TA system TA032353

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35656..36082
• Replicon: plasmid pE2863-3
• Accession: NZ_AP018805
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EL381_RS28825
• Protein ID: WP_001312861.1
• Coordinates: 35656..35814 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35858..36082 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS28780	31029..31718	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
EL381_RS28785	31905..32288	-	384	WP_001151524.1	relaxosome protein TraM	-
EL381_RS28795	32702..33211	+	510	Protein_28	transglycosylase SLT domain-containing protein	-
EL381_RS28800	33508..34329	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EL381_RS28805	34449..34736	-	288	WP_000107535.1	hypothetical protein	-
EL381_RS30050	35078..35302	+	225	WP_001427866.1	hypothetical protein	-
EL381_RS28825	35656..35814	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	35858..36082	-	225	NuclAT_0	-	Antitoxin
-	35858..36082	-	225	NuclAT_0	-	Antitoxin
-	35858..36082	-	225	NuclAT_0	-	Antitoxin
-	35858..36082	-	225	NuclAT_0	-	Antitoxin
EL381_RS30055	35894..36082	+	189	WP_001299721.1	hypothetical protein	-
EL381_RS28830	36094..36813	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EL381_RS28835	36810..37244	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
EL381_RS28840	37299..39257	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
EL381_RS28845	39316..39549	-	234	WP_000006004.1	DUF905 domain-containing protein	-
EL381_RS28850	39605..40132	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
EL381_RS28870	40605..40871	-	267	WP_071828223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..78434	78434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T32353 WP_001312861.1 NZ_AP018805:c35814-35656 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T32353 NZ_AP018805:c35814-35656 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT32353 NZ_AP018805:c36082-35858 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin