Detailed information of TA system TA032352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 33216..33927
• Replicon: plasmid pE2863-2
• Accession: NZ_AP018804
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: EL381_RS28355
• Protein ID: WP_000162415.1
• Coordinates: 33625..33927 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EL381_RS28350
• Protein ID: WP_000806445.1
• Coordinates: 33216..33554 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS28320	28510..29385	-	876	WP_032273953.1	hypothetical protein	-
EL381_RS28325	29678..30331	+	654	WP_000410954.1	hypothetical protein	-
EL381_RS28330	30660..30989	+	330	WP_000542383.1	hypothetical protein	-
EL381_RS28335	30982..32175	+	1194	WP_001112629.1	hypothetical protein	-
EL381_RS28340	32209..32937	-	729	WP_000986757.1	hypothetical protein	-
EL381_RS28345	32959..33159	-	201	WP_001022420.1	hypothetical protein	-
EL381_RS28350	33216..33554	-	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
EL381_RS28355	33625..33927	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EL381_RS28360	34090..34881	+	792	WP_000532307.1	hypothetical protein	-
EL381_RS28365	34878..35645	+	768	WP_000203293.1	hypothetical protein	-
EL381_RS28370	35649..36629	+	981	WP_000046500.1	hypothetical protein	-
EL381_RS28375	36626..37279	+	654	WP_000828869.1	hypothetical protein	-
EL381_RS28380	37339..38244	+	906	WP_001258016.1	recombination-associated protein RdgC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92925	92925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T32352 WP_000162415.1 NZ_AP018804:c33927-33625 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T32352 NZ_AP018804:c33927-33625 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT32352 WP_000806445.1 NZ_AP018804:c33554-33216 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT32352 NZ_AP018804:c33554-33216 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3