Detailed information of TA system TA032346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4181650..4182252
• Replicon: chromosome
• Accession: NZ_AP018802
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EL381_RS21420
• Protein ID: WP_000897305.1
• Coordinates: 4181941..4182252 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EL381_RS21415
• Protein ID: WP_000356397.1
• Coordinates: 4181650..4181940 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS21390	4177575..4178477	+	903	WP_000331369.1	formate dehydrogenase O subunit beta	-
EL381_RS21395	4178474..4179109	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
EL381_RS21400	4179106..4180035	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EL381_RS21405	4180366..4180608	-	243	WP_001086388.1	hypothetical protein	-
EL381_RS21410	4180827..4181045	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EL381_RS21415	4181650..4181940	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EL381_RS21420	4181941..4182252	-	312	WP_000897305.1	hypothetical protein	Toxin
EL381_RS21425	4182481..4183389	+	909	WP_001162704.1	alpha/beta hydrolase	-
EL381_RS21430	4183453..4184394	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EL381_RS21435	4184439..4184876	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EL381_RS21440	4184873..4185745	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EL381_RS21445	4185739..4186338	-	600	WP_001315111.1	glucose-1-phosphatase	-
EL381_RS21450	4186437..4187222	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T32346 WP_000897305.1 NZ_AP018802:c4182252-4181941 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T32346 NZ_AP018802:c4182252-4181941 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT32346 WP_000356397.1 NZ_AP018802:c4181940-4181650 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT32346 NZ_AP018802:c4181940-4181650 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7