Detailed information of TA system TA032342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3360556..3361283
• Replicon: chromosome
• Accession: NZ_AP018802
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: EL381_RS17410
• Protein ID: WP_000547559.1
• Coordinates: 3360972..3361283 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EL381_RS17405
• Protein ID: WP_000126294.1
• Coordinates: 3360556..3360975 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS17385	3355606..3356133	-	528	WP_001078781.1	electron transport protein HydN	-
EL381_RS17390	3356282..3357292	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
EL381_RS17395	3357552..3359009	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EL381_RS17400	3359018..3360442	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
EL381_RS17405	3360556..3360975	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EL381_RS17410	3360972..3361283	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
EL381_RS17415	3361457..3362200	-	744	WP_001369451.1	hypothetical protein	-
EL381_RS17420	3362243..3362713	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EL381_RS17425	3362706..3363116	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EL381_RS17430	3363113..3363880	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
EL381_RS17435	3363880..3364422	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EL381_RS17440	3364432..3366141	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T32342 WP_000547559.1 NZ_AP018802:c3361283-3360972 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T32342 NZ_AP018802:c3361283-3360972 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT32342 WP_000126294.1 NZ_AP018802:c3360975-3360556 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT32342 NZ_AP018802:c3360975-3360556 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163