Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2549114..2549616 Replicon chromosome
Accession NZ_AP018802
Organism Escherichia coli strain E2863

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EL381_RS13335 Protein ID WP_000767829.1
Coordinates 2549114..2549368 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EL381_RS13340 Protein ID WP_001259255.1
Coordinates 2549365..2549616 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL381_RS13305 2544129..2544356 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EL381_RS13310 2544370..2545428 - 1059 WP_000492340.1 transport protein YeeE -
EL381_RS13315 2545607..2546965 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EL381_RS13320 2546955..2547017 - 63 WP_010723108.1 membrane protein YoeI -
EL381_RS13325 2547232..2548161 - 930 WP_000803331.1 LysR family transcriptional regulator -
EL381_RS13330 2548207..2549031 - 825 WP_000754755.1 SDR family oxidoreductase -
EL381_RS13335 2549114..2549368 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EL381_RS13340 2549365..2549616 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EL381_RS13345 2549900..2549950 + 51 WP_001364200.1 his operon leader peptide -
EL381_RS13350 2550096..2550995 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EL381_RS13355 2551001..2552305 + 1305 WP_001345258.1 histidinol dehydrogenase -
EL381_RS13360 2552302..2553381 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
EL381_RS13365 2553378..2554445 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32340 WP_000767829.1 NZ_AP018802:c2549368-2549114 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32340 NZ_AP018802:c2549368-2549114 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32340 WP_001259255.1 NZ_AP018802:c2549616-2549365 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32340 NZ_AP018802:c2549616-2549365 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References