Detailed information of TA system TA032326

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1650677..1651048
• Replicon: chromosome
• Accession: NZ_AP018802
• Organism: Escherichia coli strain E2863

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EL381_RS08300
• Protein ID: WP_001443846.1
• Coordinates: 1650899..1651048 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1650677..1650855 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL381_RS08275	1646632..1648005	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EL381_RS08280	1648134..1649069	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EL381_RS08285	1649121..1650356	-	1236	WP_000040839.1	site-specific integrase	-
EL381_RS08290	1650358..1650573	-	216	WP_000079604.1	excisionase XisR	-
EL381_RS08295	1650673..1650861	-	189	WP_001302840.1	DUF1187 family protein	-
-	1650677..1650855	+	179	NuclAT_0	-	Antitoxin
-	1650677..1650855	+	179	NuclAT_0	-	Antitoxin
-	1650677..1650855	+	179	NuclAT_0	-	Antitoxin
-	1650677..1650855	+	179	NuclAT_0	-	Antitoxin
EL381_RS08300	1650899..1651048	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EL381_RS08305	1651104..1651913	-	810	WP_000166313.1	recombination protein RecT	-
EL381_RS08310	1651906..1654506	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
EL381_RS08315	1654608..1654883	-	276	WP_001344816.1	hypothetical protein	-
EL381_RS08320	1654958..1655128	-	171	WP_001352098.1	conserved protein, Rac prophage	-
EL381_RS08325	1655128..1655349	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleF / espM1 / paa / nleG7'	1649121..1739600	90479

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T32326 WP_001443846.1 NZ_AP018802:c1651048-1650899 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T32326 NZ_AP018802:c1651048-1650899 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT32326 NZ_AP018802:1650677-1650855 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin