Detailed information of TA system TA032319

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70195..70448
• Replicon: plasmid pE2855-5
• Accession: NZ_AP018801
• Organism: Escherichia coli strain E2855

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MCREHEC_RS31260
• Protein ID: WP_001312851.1
• Coordinates: 70299..70448 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70195..70254 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCREHEC_RS31335	66058..66510	+	453	Protein_85	hypothetical protein	-
MCREHEC_RS31225	66567..67780	+	1214	WP_162829202.1	IS3 family transposase	-
MCREHEC_RS31230	67821..68078	+	258	Protein_87	YadA-like family protein	-
MCREHEC_RS31235	68080..68541	+	462	WP_021553165.1	thermonuclease family protein	-
MCREHEC_RS31240	68587..68796	+	210	WP_001333231.1	hemolysin expression modulator Hha	-
MCREHEC_RS31245	68834..69424	+	591	WP_078183909.1	DUF2726 domain-containing protein	-
MCREHEC_RS31250	69579..70052	+	474	WP_078183908.1	hypothetical protein	-
-	70195..70254	-	60	NuclAT_1	-	Antitoxin
-	70195..70254	-	60	NuclAT_1	-	Antitoxin
-	70195..70254	-	60	NuclAT_1	-	Antitoxin
-	70195..70254	-	60	NuclAT_1	-	Antitoxin
MCREHEC_RS31260	70299..70448	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
MCREHEC_RS31265	70732..70989	+	258	WP_021553168.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..71289	71289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T32319 WP_001312851.1 NZ_AP018801:70299-70448 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T32319 NZ_AP018801:70299-70448 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT32319 NZ_AP018801:c70254-70195 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin