Detailed information of TA system TA032316

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31961..32230
• Replicon: plasmid pE2855-5
• Accession: NZ_AP018801
• Organism: Escherichia coli strain E2855

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MCREHEC_RS30990
• Protein ID: WP_001312861.1
• Coordinates: 32072..32230 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31961..32026 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCREHEC_RS30940	27164..27435	-	272	Protein_40	hypothetical protein	-
MCREHEC_RS30955	27733..28254	+	522	WP_021553184.1	single-stranded DNA-binding protein	-
MCREHEC_RS30965	28312..28545	+	234	WP_021553185.1	DUF905 domain-containing protein	-
MCREHEC_RS30970	28609..30573	+	1965	WP_078183929.1	ParB/RepB/Spo0J family partition protein	-
MCREHEC_RS30975	30642..31076	+	435	WP_000845908.1	conjugation system SOS inhibitor PsiB	-
MCREHEC_RS30980	31073..31792	+	720	WP_078183928.1	plasmid SOS inhibition protein A	-
MCREHEC_RS30985	31804..31992	-	189	WP_032145107.1	hypothetical protein	-
-	31804..32028	+	225	NuclAT_0	-	-
-	31804..32028	+	225	NuclAT_0	-	-
-	31804..32028	+	225	NuclAT_0	-	-
-	31804..32028	+	225	NuclAT_0	-	-
-	31961..32026	+	66	-	-	Antitoxin
MCREHEC_RS30990	32072..32230	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
MCREHEC_RS31005	32898..33101	+	204	Protein_48	single-stranded DNA-binding protein	-
MCREHEC_RS31010	33124..33447	+	324	WP_000533253.1	hypothetical protein	-
MCREHEC_RS31015	33506..33748	+	243	WP_000540591.1	hypothetical protein	-
MCREHEC_RS31040	34671..34967	+	297	WP_021553188.1	hypothetical protein	-
MCREHEC_RS31045	35078..35899	+	822	WP_021553189.1	DUF945 domain-containing protein	-
MCREHEC_RS31050	36195..36704	-	510	WP_021553190.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..71289	71289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T32316 WP_001312861.1 NZ_AP018801:32072-32230 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T32316 NZ_AP018801:32072-32230 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT32316 NZ_AP018801:31961-32026 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin