Detailed information of TA system TA032311

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35660..36086
• Replicon: plasmid pE2855-4
• Accession: NZ_AP018800
• Organism: Escherichia coli strain E2855

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MCREHEC_RS30390
• Protein ID: WP_001312861.1
• Coordinates: 35660..35818 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35862..36086 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCREHEC_RS30345	31030..31719	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
MCREHEC_RS30350	31906..32289	-	384	WP_001151524.1	relaxosome protein TraM	-
MCREHEC_RS30360	32703..33212	+	510	Protein_29	transglycosylase SLT domain-containing protein	-
MCREHEC_RS30365	33509..34330	-	822	WP_001234469.1	DUF945 domain-containing protein	-
MCREHEC_RS30370	34451..34738	-	288	WP_000107535.1	hypothetical protein	-
MCREHEC_RS31660	35082..35306	+	225	WP_001427866.1	hypothetical protein	-
MCREHEC_RS30390	35660..35818	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
MCREHEC_RS31665	35898..36086	+	189	WP_001299721.1	hypothetical protein	-
MCREHEC_RS30395	36098..36817	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
MCREHEC_RS30400	36814..37248	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
MCREHEC_RS30405	37303..39261	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
MCREHEC_RS30410	39320..39553	-	234	WP_000006004.1	DUF905 domain-containing protein	-
MCREHEC_RS30415	39609..40136	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
MCREHEC_RS31670	40609..40887	-	279	WP_001371319.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..81004	81004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T32311 WP_001312861.1 NZ_AP018800:c35818-35660 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T32311 NZ_AP018800:c35818-35660 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT32311 NZ_AP018800:c36086-35862 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin