Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4018188..4018987 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MCREHEC_RS20960 Protein ID WP_000347273.1
Coordinates 4018523..4018987 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MCREHEC_RS20955 Protein ID WP_001307405.1
Coordinates 4018188..4018523 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS20940 4013973..4014743 - 771 WP_001058225.1 2-dehydro-3-deoxyglucarate aldolase -
MCREHEC_RS20945 4014759..4016093 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MCREHEC_RS20950 4016468..4018039 + 1572 WP_001273741.1 galactarate dehydratase -
MCREHEC_RS20955 4018188..4018523 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MCREHEC_RS20960 4018523..4018987 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MCREHEC_RS20965 4019042..4019851 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
MCREHEC_RS20970 4020100..4021380 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MCREHEC_RS20975 4021403..4021876 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MCREHEC_RS20980 4021887..4022666 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
MCREHEC_RS20985 4022656..4023534 + 879 WP_001341890.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
MCREHEC_RS20990 4023552..4023986 + 435 WP_000948822.1 PTS sugar transporter subunit IIA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4007532..4018987 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T32305 WP_000347273.1 NZ_AP018796:4018523-4018987 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T32305 NZ_AP018796:4018523-4018987 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT32305 WP_001307405.1 NZ_AP018796:4018188-4018523 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT32305 NZ_AP018796:4018188-4018523 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References