32302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3472456..3473183 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name higB Uniprot ID I2UEW4
Locus tag MCREHEC_RS18225 Protein ID WP_000547559.1
Coordinates 3472872..3473183 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MCREHEC_RS18220 Protein ID WP_000126294.1
Coordinates 3472456..3472875 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS18200 3467506..3468033 - 528 WP_001078781.1 electron transport protein HydN -
MCREHEC_RS18205 3468182..3469192 - 1011 WP_001377591.1 DNA-binding transcriptional regulator AscG -
MCREHEC_RS18210 3469452..3470909 + 1458 WP_001107856.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
MCREHEC_RS18215 3470918..3472342 + 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
MCREHEC_RS18220 3472456..3472875 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
MCREHEC_RS18225 3472872..3473183 - 312 WP_000547559.1 type II toxin-antitoxin system HigB family toxin Toxin
MCREHEC_RS18230 3473357..3474100 - 744 WP_001369451.1 hypothetical protein -
MCREHEC_RS18235 3474143..3474613 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
MCREHEC_RS18240 3474606..3475016 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
MCREHEC_RS18245 3475013..3475780 - 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
MCREHEC_RS18250 3475780..3476322 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
MCREHEC_RS18255 3476332..3478041 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12450.11 Da        Isoelectric Point: 9.7249

>T32302 WP_000547559.1 NZ_AP018796:c3473183-3472872 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T32302 NZ_AP018796:c3473183-3472872 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT32302 WP_000126294.1 NZ_AP018796:c3472875-3472456 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT32302 NZ_AP018796:c3472875-3472456 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0F3V163


Antitoxin

Source ID Structure

References