32300

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2582191..2582693 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MCREHEC_RS13540 Protein ID WP_000767829.1
Coordinates 2582191..2582445 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MCREHEC_RS13545 Protein ID WP_001259255.1
Coordinates 2582442..2582693 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS13510 2577206..2577433 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
MCREHEC_RS13515 2577447..2578505 - 1059 WP_000492340.1 transport protein YeeE -
MCREHEC_RS13520 2578684..2580042 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MCREHEC_RS13525 2580032..2580094 - 63 WP_010723108.1 membrane protein YoeI -
MCREHEC_RS13530 2580309..2581238 - 930 WP_000803331.1 LysR family transcriptional regulator -
MCREHEC_RS13535 2581284..2582108 - 825 WP_000754755.1 SDR family oxidoreductase -
MCREHEC_RS13540 2582191..2582445 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MCREHEC_RS13545 2582442..2582693 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MCREHEC_RS13550 2582977..2583027 + 51 WP_001364200.1 his operon leader peptide -
MCREHEC_RS13555 2583173..2584072 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MCREHEC_RS13560 2584078..2585382 + 1305 WP_001345258.1 histidinol dehydrogenase -
MCREHEC_RS13565 2585379..2586458 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
MCREHEC_RS13570 2586455..2587522 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32300 WP_000767829.1 NZ_AP018796:c2582445-2582191 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32300 NZ_AP018796:c2582445-2582191 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32300 WP_001259255.1 NZ_AP018796:c2582693-2582442 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32300 NZ_AP018796:c2582693-2582442 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References