Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2582191..2582693 Replicon chromosome
Accession NZ_AP018796
Organism Escherichia coli strain E2855

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MCREHEC_RS13540 Protein ID WP_000767829.1
Coordinates 2582191..2582445 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MCREHEC_RS13545 Protein ID WP_001259255.1
Coordinates 2582442..2582693 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCREHEC_RS13510 2577206..2577433 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
MCREHEC_RS13515 2577447..2578505 - 1059 WP_000492340.1 transport protein YeeE -
MCREHEC_RS13520 2578684..2580042 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MCREHEC_RS13525 2580032..2580094 - 63 WP_010723108.1 membrane protein YoeI -
MCREHEC_RS13530 2580309..2581238 - 930 WP_000803331.1 LysR family transcriptional regulator -
MCREHEC_RS13535 2581284..2582108 - 825 WP_000754755.1 SDR family oxidoreductase -
MCREHEC_RS13540 2582191..2582445 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MCREHEC_RS13545 2582442..2582693 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MCREHEC_RS13550 2582977..2583027 + 51 WP_001364200.1 his operon leader peptide -
MCREHEC_RS13555 2583173..2584072 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MCREHEC_RS13560 2584078..2585382 + 1305 WP_001345258.1 histidinol dehydrogenase -
MCREHEC_RS13565 2585379..2586458 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
MCREHEC_RS13570 2586455..2587522 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32300 WP_000767829.1 NZ_AP018796:c2582445-2582191 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32300 NZ_AP018796:c2582445-2582191 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32300 WP_001259255.1 NZ_AP018796:c2582693-2582442 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32300 NZ_AP018796:c2582693-2582442 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References