Detailed information of TA system TA032272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 702813..703651
• Replicon: chromosome
• Accession: NZ_AP018795
• Organism: Acidithiobacillus ferridurans strain JCM 18981

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AFERRID_RS03625
• Protein ID: WP_126604370.1
• Coordinates: 702813..703325 (-)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: AFERRID_RS03630
• Protein ID: WP_113525968.1
• Coordinates: 703322..703651 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFERRID_RS03595	697935..698882	+	948	WP_113525973.1	ribose-phosphate pyrophosphokinase	-
AFERRID_RS03605	699432..700595	-	1164	WP_113525972.1	outer membrane beta-barrel protein	-
AFERRID_RS03610	701402..701596	+	195	WP_126604369.1	hypothetical protein	-
AFERRID_RS03615	701732..701884	-	153	WP_197722476.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AFERRID_RS03620	702047..702673	+	627	WP_172959400.1	trimeric intracellular cation channel family protein	-
AFERRID_RS03625	702813..703325	-	513	WP_126604370.1	type II toxin-antitoxin system YhaV family toxin	Toxin
AFERRID_RS03630	703322..703651	-	330	WP_113525968.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
AFERRID_RS03635	704234..704875	+	642	WP_192796469.1	hypothetical protein	-
AFERRID_RS15270	705119..705313	+	195	WP_181710098.1	hypothetical protein	-
AFERRID_RS03640	705716..705835	-	120	Protein_701	IS3 family transposase	-
AFERRID_RS03650	706620..706838	+	219	WP_113525967.1	hypothetical protein	-
AFERRID_RS03655	706890..707927	+	1038	WP_113525966.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pentatricopeptide repeat (124-143)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 19612.25 Da
• Isoelectric Point: 9.7630

>T32272 WP_126604370.1 NZ_AP018795:c703325-702813 [Acidithiobacillus ferridurans]
ISAGKPEPLVIHGWTVFAHPLFLAQIEALARQVETLKQKDPAGYVKKNASKRLAAITKLAFDVIPQDPTRPEYRQGGTLG
DDHKHWFRAKFFQQYRLFFRYHAPSKVIVYAWVNDEDSKRAFERSDDAYRVFRKMLESGHPPDDWDQLLAEAQKESQRMQ
ESLARAAAAQ

Nucleotide

• Length: 513 bp

>T32272 NZ_AP018795:c703325-702813 [Acidithiobacillus ferridurans]
ATAAGTGCAGGGAAGCCTGAGCCCTTGGTCATTCATGGCTGGACGGTCTTCGCCCATCCACTGTTCCTGGCGCAGATTGA
AGCGCTGGCGCGGCAGGTCGAGACTCTGAAGCAGAAGGACCCGGCCGGGTACGTAAAAAAGAACGCCAGCAAACGGCTGG
CCGCGATCACCAAACTGGCGTTCGATGTCATCCCGCAAGATCCGACGCGACCGGAGTATCGCCAGGGCGGGACGCTCGGT
GACGATCACAAGCACTGGTTTCGGGCCAAGTTCTTCCAGCAGTACCGACTATTCTTCCGCTACCATGCCCCGAGCAAGGT
GATCGTCTACGCCTGGGTGAATGACGAAGACAGCAAACGTGCCTTTGAGCGCAGCGACGACGCCTACCGGGTGTTCCGCA
AGATGCTGGAAAGCGGGCATCCGCCGGATGACTGGGATCAGCTCCTGGCTGAGGCGCAGAAAGAATCGCAGCGGATGCAG
GAATCATTGGCCAGGGCGGCAGCAGCGCAGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11844.33 Da
• Isoelectric Point: 4.5282

>AT32272 WP_113525968.1 NZ_AP018795:c703651-703322 [Acidithiobacillus ferridurans]
MAATLEVESTLTDRYQTTVPETVRRALKLGKRDKIHYTIRPSGEVVLTRVQASDGDDPVLGQFLGFLAADITRHPERLQA
VDAGLVQRLQSLVGGVDVDLDAALSADDE

Nucleotide

• Length: 330 bp

>AT32272 NZ_AP018795:c703651-703322 [Acidithiobacillus ferridurans]
ATGGCCGCGACCCTCGAAGTCGAATCCACTCTCACCGATCGCTACCAGACCACGGTGCCGGAAACCGTGCGTCGTGCCCT
CAAGCTGGGCAAGCGCGACAAGATCCACTACACCATTCGCCCCAGCGGAGAGGTGGTGCTGACGCGCGTCCAAGCATCCG
ACGGTGACGACCCTGTGCTCGGTCAGTTTCTCGGCTTCCTGGCCGCCGATATCACTCGCCATCCTGAGCGGCTGCAAGCT
GTGGATGCCGGCCTCGTCCAACGCCTCCAGTCGCTGGTCGGCGGCGTCGATGTCGATCTCGATGCCGCCCTGTCGGCAGA
CGATGAATAA