Detailed information of TA system TA032263

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36733..37003
• Replicon: plasmid pSK1144
• Accession: NZ_AP018785
• Organism: Escherichia coli strain SK1144

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DAECLI1_RS25160
• Protein ID: WP_001312861.1
• Coordinates: 36845..37003 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36733..36796 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAECLI1_RS25135	32503..33030	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
DAECLI1_RS25140	33088..33321	+	234	WP_000006003.1	DUF905 family protein	-
DAECLI1_RS25145	33382..35346	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
DAECLI1_RS25150	35415..35849	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DAECLI1_RS25155	35846..36565	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36577..36801	+	225	NuclAT_0	-	-
-	36577..36801	+	225	NuclAT_0	-	-
-	36577..36801	+	225	NuclAT_0	-	-
-	36577..36801	+	225	NuclAT_0	-	-
DAECLI1_RS26305	36586..36765	-	180	WP_001309233.1	hypothetical protein	-
-	36733..36796	-	64	-	-	Antitoxin
DAECLI1_RS25160	36845..37003	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DAECLI1_RS25180	37919..38215	+	297	WP_001272251.1	hypothetical protein	-
DAECLI1_RS25185	38326..39147	+	822	WP_021549731.1	DUF945 domain-containing protein	-
DAECLI1_RS25190	39444..40091	-	648	Protein_48	transglycosylase SLT domain-containing protein	-
DAECLI1_RS25195	40367..40750	+	384	WP_001151529.1	relaxosome protein TraM	-
DAECLI1_RS25200	40942..41589	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
DAECLI1_RS25205	41697..41936	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..169561	169561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T32263 WP_001312861.1 NZ_AP018785:36845-37003 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T32263 NZ_AP018785:36845-37003 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT32263 NZ_AP018785:c36796-36733 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin