Detailed information of TA system TA032234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1369523..1370162
• Replicon: chromosome
• Accession: NZ_AP018783
• Organism: Haemophilus influenzae strain CHBN-V-3 isolate 15H03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J71_RS06825
• Protein ID: WP_005650215.1
• Coordinates: 1369523..1369828 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J71_RS06830
• Protein ID: WP_005650217.1
• Coordinates: 1369839..1370162 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J71_RS06805 (CHBNV3_13020)	1364953..1366992	-	2040	WP_005646675.1	excinuclease ABC subunit UvrB	-
K6J71_RS06815 (CHBNV3_13030)	1367607..1368575	-	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
K6J71_RS06820 (CHBNV3_13040)	1368578..1369198	-	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
K6J71_RS06825 (CHBNV3_13050)	1369523..1369828	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J71_RS06830 (CHBNV3_13060)	1369839..1370162	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J71_RS06835 (CHBNV3_13070)	1370325..1371995	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J71_RS06840 (CHBNV3_13080)	1372057..1372398	+	342	WP_005666735.1	SirB2 family protein	-
K6J71_RS06845 (CHBNV3_13090)	1372403..1374373	+	1971	WP_005666737.1	GNAT family N-acetyltransferase	-
K6J71_RS06850 (CHBNV3_13100)	1374370..1374543	+	174	WP_005637028.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32234 WP_005650215.1 NZ_AP018783:1369523-1369828 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32234 NZ_AP018783:1369523-1369828 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32234 WP_005650217.1 NZ_AP018783:1369839-1370162 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32234 NZ_AP018783:1369839-1370162 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70