Detailed information of TA system TA032225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1222264..1222903
• Replicon: chromosome
• Accession: NZ_AP018782
• Organism: Haemophilus influenzae strain CHBN-V-2 isolate 13H37

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J59_RS06040
• Protein ID: WP_005650215.1
• Coordinates: 1222264..1222569 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J59_RS06045
• Protein ID: WP_005650217.1
• Coordinates: 1222580..1222903 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J59_RS06020 (CHBNV2_11390)	1217694..1219733	-	2040	WP_005646675.1	excinuclease ABC subunit UvrB	-
K6J59_RS06030 (CHBNV2_11400)	1220348..1221316	-	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
K6J59_RS06035 (CHBNV2_11410)	1221319..1221939	-	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
K6J59_RS06040 (CHBNV2_11420)	1222264..1222569	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J59_RS06045 (CHBNV2_11430)	1222580..1222903	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J59_RS06050 (CHBNV2_11440)	1223066..1224736	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J59_RS06055 (CHBNV2_11450)	1224798..1225139	+	342	WP_005666735.1	SirB2 family protein	-
K6J59_RS06060 (CHBNV2_11460)	1225144..1227114	+	1971	WP_005666737.1	GNAT family N-acetyltransferase	-
K6J59_RS06065 (CHBNV2_11470)	1227111..1227284	+	174	WP_005637028.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32225 WP_005650215.1 NZ_AP018782:1222264-1222569 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32225 NZ_AP018782:1222264-1222569 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32225 WP_005650217.1 NZ_AP018782:1222580-1222903 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32225 NZ_AP018782:1222580-1222903 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70