Detailed information of TA system TA032217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1342683..1343322
• Replicon: chromosome
• Accession: NZ_AP018781
• Organism: Haemophilus influenzae strain CHBN-V-1 isolate 13H43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: CHBNTHi_RS06680
• Protein ID: WP_005650215.1
• Coordinates: 1342683..1342988 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CHBNTHi_RS06685
• Protein ID: WP_005650217.1
• Coordinates: 1342999..1343322 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHBNTHi_RS06660 (CHBNV1_12630)	1338114..1340153	-	2040	WP_075985258.1	excinuclease ABC subunit UvrB	-
CHBNTHi_RS06670 (CHBNV1_12640)	1340767..1341735	-	969	WP_005650211.1	zinc transporter permease subunit ZevB	-
CHBNTHi_RS06675 (CHBNV1_12650)	1341738..1342358	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
CHBNTHi_RS06680 (CHBNV1_12660)	1342683..1342988	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHBNTHi_RS06685 (CHBNV1_12670)	1342999..1343322	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
CHBNTHi_RS06690 (CHBNV1_12680)	1343485..1345155	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
CHBNTHi_RS06695 (CHBNV1_12690)	1345217..1345558	+	342	WP_005666735.1	SirB2 family protein	-
CHBNTHi_RS06700 (CHBNV1_12700)	1345563..1347533	+	1971	WP_005666737.1	GNAT family N-acetyltransferase	-
CHBNTHi_RS06705 (CHBNV1_12710)	1347530..1347703	+	174	WP_005637028.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32217 WP_005650215.1 NZ_AP018781:1342683-1342988 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32217 NZ_AP018781:1342683-1342988 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32217 WP_005650217.1 NZ_AP018781:1342999-1343322 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32217 NZ_AP018781:1342999-1343322 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70