Detailed information of TA system TA032205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 341347..342695
• Replicon: chromosome
• Accession: NZ_AP018780
• Organism: Haemophilus influenzae strain CHBN-IV-1 isolate 13H36

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J73_RS01700
• Protein ID: WP_221253265.1
• Coordinates: 341664..342695 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2S9R6P7
• Locus tag: K6J73_RS01695
• Protein ID: WP_005650823.1
• Coordinates: 341347..341667 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J73_RS01655 (CHBNIV1_03190)	336767..337045	+	279	WP_005665661.1	cell division protein FtsB	-
K6J73_RS01660 (CHBNIV1_03200)	337045..337722	+	678	WP_221253264.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J73_RS01665 (CHBNIV1_03210)	337719..338195	+	477	WP_014551115.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J73_RS01670 (CHBNIV1_03220)	338505..338939	-	435	WP_014551116.1	D-aminoacyl-tRNA deacylase	-
K6J73_RS01675 (CHBNIV1_03230)	338936..339376	-	441	WP_020910205.1	FMN-binding protein MioC	-
K6J73_RS01680 (CHBNIV1_03240)	339470..339688	-	219	WP_005650820.1	cell division protein ZapB	-
K6J73_RS01685 (CHBNIV1_03250)	339850..340851	+	1002	WP_105886407.1	class II fructose-bisphosphatase	-
K6J73_RS01690 (CHBNIV1_03260)	341051..341359	+	309	WP_005661200.1	helix-turn-helix transcriptional regulator	-
K6J73_RS01695 (CHBNIV1_03270)	341347..341667	+	321	WP_005650823.1	HipA N-terminal domain-containing protein	Antitoxin
K6J73_RS01700 (CHBNIV1_03280)	341664..342695	+	1032	WP_221253265.1	HipA domain-containing protein	Toxin
K6J73_RS01705 (CHBNIV1_03290)	342779..344437	-	1659	WP_221253266.1	thiol reductant ABC exporter subunit CydC	-
K6J73_RS01710 (CHBNIV1_03300)	344430..346175	-	1746	WP_221253267.1	ABC transporter ATP-binding protein/permease	-
K6J73_RS09360	346542..346648	+	107	Protein_336	PT domain-containing protein	-
K6J73_RS09365	346626..346697	+	72	Protein_337	PT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39869.52 Da
• Isoelectric Point: 6.7803

>T32205 WP_221253265.1 NZ_AP018780:341664-342695 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSGYSTKGLHYLTGNKNFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDEHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQKPMHQEQLDGAMNIRDK
YGKIGADNEQYVSYEQIAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEIIQEKEKVEQIYSQSFMPQPH
IDCVLKTYRKRLALLNVLHEPEL

Nucleotide

• Length: 1032 bp

>T32205 NZ_AP018780:341664-342695 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGGTTACAGCACAAAAGGTTTACATTA
TCTTACAGGCAATAAAAATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGAGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACACGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGATGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAGCAAAAACCAATGCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTGCAGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTGCAGCACACTGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTGTCAGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCCTTGCCATTGTTAGCGCGAGAAGAAGGTAATGCCACACTAGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATTCCTGAAATTATACAAGAAAAGGAAAAAGTAGAGCAGATTTATTCACAATCCTTTATGCCGCAGCCACAT
ATTGATTGCGTGCTGAAAACTTATCGCAAGCGTTTAGCATTACTTAACGTATTACATGAACCTGAACTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12404.04 Da
• Isoelectric Point: 5.8850

>AT32205 WP_005650823.1 NZ_AP018780:341347-341667 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHHGFHFSYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
EGDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32205 NZ_AP018780:341347-341667 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCATGGTTT
TCATTTTTCTTACAACCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGTCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCCCTCGTACCCGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAGGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGCGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: A0A2S9R6P7