Detailed information of TA system TA032201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1223048..1223687
• Replicon: chromosome
• Accession: NZ_AP018779
• Organism: Haemophilus influenzae strain CHBN-III-8 isolate 10H15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J62_RS06005
• Protein ID: WP_005650215.1
• Coordinates: 1223048..1223353 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J62_RS06010
• Protein ID: WP_005650217.1
• Coordinates: 1223364..1223687 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J62_RS05985 (CHBNIII8_11330)	1218478..1220517	-	2040	WP_105873737.1	excinuclease ABC subunit UvrB	-
K6J62_RS05995 (CHBNIII8_11340)	1221132..1222100	-	969	WP_110432018.1	zinc transporter permease subunit ZevB	-
K6J62_RS06000 (CHBNIII8_11350)	1222103..1222723	-	621	WP_110432017.1	zinc transporter binding subunit ZevA	-
K6J62_RS06005 (CHBNIII8_11360)	1223048..1223353	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J62_RS06010 (CHBNIII8_11370)	1223364..1223687	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J62_RS06015 (CHBNIII8_11380)	1223850..1225520	+	1671	WP_110432016.1	energy-dependent translational throttle protein EttA	-
K6J62_RS06020 (CHBNIII8_11390)	1225582..1225923	+	342	WP_005652996.1	SirB2 family protein	-
K6J62_RS06025 (CHBNIII8_11400)	1225928..1227898	+	1971	WP_110432015.1	GNAT family N-acetyltransferase	-
K6J62_RS06030 (CHBNIII8_11410)	1227895..1228068	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32201 WP_005650215.1 NZ_AP018779:1223048-1223353 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32201 NZ_AP018779:1223048-1223353 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32201 WP_005650217.1 NZ_AP018779:1223364-1223687 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32201 NZ_AP018779:1223364-1223687 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70