Detailed information of TA system TA032196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1265337..1265976
• Replicon: chromosome
• Accession: NZ_AP018778
• Organism: Haemophilus influenzae strain CHBN-III-7 isolate 15H01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J60_RS06385
• Protein ID: WP_005650215.1
• Coordinates: 1265337..1265642 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J60_RS06390
• Protein ID: WP_005650217.1
• Coordinates: 1265653..1265976 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J60_RS06365 (CHBNIII7_11930)	1260768..1262807	-	2040	WP_075985258.1	excinuclease ABC subunit UvrB	-
K6J60_RS06375 (CHBNIII7_11940)	1263421..1264389	-	969	WP_221267221.1	zinc transporter permease subunit ZevB	-
K6J60_RS06380 (CHBNIII7_11950)	1264392..1265012	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
K6J60_RS06385 (CHBNIII7_11960)	1265337..1265642	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J60_RS06390 (CHBNIII7_11970)	1265653..1265976	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J60_RS06395 (CHBNIII7_11980)	1266139..1267809	+	1671	WP_118807668.1	energy-dependent translational throttle protein EttA	-
K6J60_RS06400 (CHBNIII7_11990)	1267871..1268212	+	342	WP_192885203.1	SirB2 family protein	-
K6J60_RS06405 (CHBNIII7_12000)	1268217..1270061	+	1845	WP_221267222.1	GNAT family N-acetyltransferase	-
K6J60_RS06410 (CHBNIII7_12010)	1270058..1270231	+	174	WP_005637028.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32196 WP_005650215.1 NZ_AP018778:1265337-1265642 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32196 NZ_AP018778:1265337-1265642 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32196 WP_005650217.1 NZ_AP018778:1265653-1265976 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32196 NZ_AP018778:1265653-1265976 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70