Detailed information of TA system TA032183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1234815..1235454
• Replicon: chromosome
• Accession: NZ_AP018776
• Organism: Haemophilus influenzae strain CHBN-III-5 isolate 13H30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J67_RS06035
• Protein ID: WP_005650215.1
• Coordinates: 1234815..1235120 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J67_RS06040
• Protein ID: WP_005650217.1
• Coordinates: 1235131..1235454 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J67_RS06015 (CHBNIII5_11360)	1230244..1232283	-	2040	WP_005687320.1	excinuclease ABC subunit UvrB	-
K6J67_RS06025 (CHBNIII5_11370)	1232899..1233867	-	969	WP_005657582.1	zinc transporter permease subunit ZevB	-
K6J67_RS06030 (CHBNIII5_11380)	1233870..1234490	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
K6J67_RS06035 (CHBNIII5_11390)	1234815..1235120	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J67_RS06040 (CHBNIII5_11400)	1235131..1235454	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J67_RS06045 (CHBNIII5_11410)	1235617..1237287	+	1671	WP_050822628.1	energy-dependent translational throttle protein EttA	-
K6J67_RS06050 (CHBNIII5_11420)	1237349..1237690	+	342	WP_050822626.1	SirB2 family protein	-
K6J67_RS06055 (CHBNIII5_11430)	1237695..1239539	+	1845	WP_050822624.1	GNAT family N-acetyltransferase	-
K6J67_RS06060 (CHBNIII5_11440)	1239536..1239709	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32183 WP_005650215.1 NZ_AP018776:1234815-1235120 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32183 NZ_AP018776:1234815-1235120 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32183 WP_005650217.1 NZ_AP018776:1235131-1235454 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32183 NZ_AP018776:1235131-1235454 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70