Detailed information of TA system TA032174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1239932..1240571
• Replicon: chromosome
• Accession: NZ_AP018775
• Organism: Haemophilus influenzae strain CHBN-III-4 isolate 12H35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J64_RS06100
• Protein ID: WP_005650215.1
• Coordinates: 1239932..1240237 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J64_RS06105
• Protein ID: WP_005650217.1
• Coordinates: 1240248..1240571 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J64_RS06080 (CHBNIII4_11460)	1235363..1237402	-	2040	WP_005687320.1	excinuclease ABC subunit UvrB	-
K6J64_RS06090 (CHBNIII4_11470)	1238016..1238984	-	969	WP_014550701.1	zinc transporter permease subunit ZevB	-
K6J64_RS06095 (CHBNIII4_11480)	1238987..1239607	-	621	WP_014550702.1	zinc transporter binding subunit ZevA	-
K6J64_RS06100 (CHBNIII4_11490)	1239932..1240237	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J64_RS06105 (CHBNIII4_11500)	1240248..1240571	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J64_RS06110 (CHBNIII4_11510)	1240735..1242405	+	1671	WP_005663485.1	energy-dependent translational throttle protein EttA	-
K6J64_RS06115 (CHBNIII4_11520)	1242467..1242808	+	342	WP_005652996.1	SirB2 family protein	-
K6J64_RS06120 (CHBNIII4_11530)	1242813..1244783	+	1971	WP_014550704.1	GNAT family N-acetyltransferase	-
K6J64_RS06125 (CHBNIII4_11540)	1244780..1244953	+	174	WP_005652999.1	DUF5363 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32174 WP_005650215.1 NZ_AP018775:1239932-1240237 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32174 NZ_AP018775:1239932-1240237 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32174 WP_005650217.1 NZ_AP018775:1240248-1240571 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32174 NZ_AP018775:1240248-1240571 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70