Detailed information of TA system TA032170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 356594..357942
• Replicon: chromosome
• Accession: NZ_AP018775
• Organism: Haemophilus influenzae strain CHBN-III-4 isolate 12H35

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J64_RS01815
• Protein ID: WP_044345221.1
• Coordinates: 356911..357942 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2R3FM14
• Locus tag: K6J64_RS01810
• Protein ID: WP_005687922.1
• Coordinates: 356594..356914 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J64_RS01770 (CHBNIII4_03420)	352022..352300	+	279	WP_005665661.1	cell division protein FtsB	-
K6J64_RS01775 (CHBNIII4_03430)	352300..352977	+	678	WP_014551114.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J64_RS01780 (CHBNIII4_03440)	352974..353450	+	477	WP_014551115.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J64_RS01785 (CHBNIII4_03450)	353752..354186	-	435	WP_005687917.1	D-aminoacyl-tRNA deacylase	-
K6J64_RS01790 (CHBNIII4_03460)	354183..354623	-	441	WP_005687918.1	FMN-binding protein MioC	-
K6J64_RS01795 (CHBNIII4_03470)	354717..354935	-	219	WP_005650820.1	cell division protein ZapB	-
K6J64_RS01800 (CHBNIII4_03480)	355097..356098	+	1002	WP_005687920.1	class II fructose-bisphosphatase	-
K6J64_RS01805 (CHBNIII4_03490)	356298..356606	+	309	WP_005661200.1	helix-turn-helix transcriptional regulator	-
K6J64_RS01810 (CHBNIII4_03500)	356594..356914	+	321	WP_005687922.1	HipA N-terminal domain-containing protein	Antitoxin
K6J64_RS01815 (CHBNIII4_03510)	356911..357942	+	1032	WP_044345221.1	HipA domain-containing protein	Toxin
K6J64_RS01820 (CHBNIII4_03520)	358026..359684	-	1659	WP_048946893.1	thiol reductant ABC exporter subunit CydC	-
K6J64_RS01825 (CHBNIII4_03530)	359677..361422	-	1746	WP_048946894.1	ABC transporter ATP-binding protein/permease	-
K6J64_RS08975	361778..361933	+	156	WP_233993456.1	PT domain-containing protein	-
K6J64_RS08980	361869..362288	+	420	Protein_362	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39860.37 Da
• Isoelectric Point: 6.5052

>T32170 WP_044345221.1 NZ_AP018775:356911-357942 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSDYSTKGLHYLTGNKHFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDGHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQTEIHQEQLDGAMNIRDK
YGKIGADNEQYVSYEQIAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEITQEKEKVEQIYSQSFMPQPH
IDCVLKTYRKRLTLLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T32170 NZ_AP018775:356911-357942 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGACTACAGCACAAAAGGTTTACATTA
CCTTACAGGCAATAAACATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGGGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACACGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGATGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAACAAACAGAGATTCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTGCAGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTGCAGCACACTGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTGTCGGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCTTTGCCATTGTTAGCGCGAGAAGAAGGTAATGCCACACTAGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATTCCTGAAATTACACAAGAAAAGGAAAAAGTAGAGCAGATTTATTCACAATCCTTTATGCCGCAGCCACAT
ATTGATTGCGTGCTGAAAACTTATCGCAAGCGTTTAACGTTACTTAACGTATTAAATGAACCTGAGCTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12407.09 Da
• Isoelectric Point: 5.9670

>AT32170 WP_005687922.1 NZ_AP018775:356594-356914 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHRGFHFAYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
EGDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32170 NZ_AP018775:356594-356914 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCGTGGTTT
TCATTTTGCTTACAATCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGCCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCGCTCGTCCCAGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAGGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGTGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: A0A2R3FM14