Detailed information of TA system TA032165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1465575..1466214
• Replicon: chromosome
• Accession: NZ_AP018774
• Organism: Haemophilus influenzae strain CHBN-III-3 isolate 13H42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J70_RS07155
• Protein ID: WP_005650215.1
• Coordinates: 1465909..1466214 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J70_RS07150
• Protein ID: WP_005650217.1
• Coordinates: 1465575..1465898 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J70_RS07130 (CHBNIII3_13570)	1461194..1461367	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
K6J70_RS07135 (CHBNIII3_13580)	1461364..1463334	-	1971	WP_011961904.1	GNAT family N-acetyltransferase	-
K6J70_RS07140 (CHBNIII3_13590)	1463339..1463680	-	342	WP_005652996.1	SirB2 family protein	-
K6J70_RS07145 (CHBNIII3_13600)	1463742..1465412	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J70_RS07150 (CHBNIII3_13610)	1465575..1465898	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J70_RS07155 (CHBNIII3_13620)	1465909..1466214	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J70_RS07160 (CHBNIII3_13630)	1466539..1467159	+	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
K6J70_RS07165 (CHBNIII3_13640)	1467162..1468130	+	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
K6J70_RS07175 (CHBNIII3_13650)	1468745..1470784	+	2040	WP_005687320.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32165 WP_005650215.1 NZ_AP018774:c1466214-1465909 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32165 NZ_AP018774:c1466214-1465909 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32165 WP_005650217.1 NZ_AP018774:c1465898-1465575 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32165 NZ_AP018774:c1465898-1465575 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70