Detailed information of TA system TA032155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1243140..1243779
• Replicon: chromosome
• Accession: NZ_AP018773
• Organism: Haemophilus influenzae strain CHBN-III-2 isolate 13H39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J68_RS06040
• Protein ID: WP_005650215.1
• Coordinates: 1243140..1243445 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J68_RS06045
• Protein ID: WP_005650217.1
• Coordinates: 1243456..1243779 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J68_RS06020 (CHBNIII2_11420)	1238570..1240609	-	2040	WP_005687320.1	excinuclease ABC subunit UvrB	-
K6J68_RS06030 (CHBNIII2_11430)	1241224..1242192	-	969	WP_105197835.1	zinc transporter permease subunit ZevB	-
K6J68_RS06035 (CHBNIII2_11440)	1242195..1242815	-	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
K6J68_RS06040 (CHBNIII2_11450)	1243140..1243445	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J68_RS06045 (CHBNIII2_11460)	1243456..1243779	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J68_RS06050 (CHBNIII2_11470)	1243942..1245612	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J68_RS06055 (CHBNIII2_11480)	1245674..1246015	+	342	WP_005652996.1	SirB2 family protein	-
K6J68_RS06060 (CHBNIII2_11490)	1246020..1247990	+	1971	WP_011272701.1	GNAT family N-acetyltransferase	-
K6J68_RS06065 (CHBNIII2_11500)	1247987..1248160	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32155 WP_005650215.1 NZ_AP018773:1243140-1243445 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32155 NZ_AP018773:1243140-1243445 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32155 WP_005650217.1 NZ_AP018773:1243456-1243779 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32155 NZ_AP018773:1243456-1243779 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70