Detailed information of TA system TA032146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1242478..1243117
• Replicon: chromosome
• Accession: NZ_AP018772
• Organism: Haemophilus influenzae strain CHBN-III-1 isolate 15H02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J58_RS06020
• Protein ID: WP_005650215.1
• Coordinates: 1242478..1242783 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J58_RS06025
• Protein ID: WP_005650217.1
• Coordinates: 1242794..1243117 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J58_RS06000 (CHBNIII1_11320)	1237908..1239947	-	2040	WP_005687320.1	excinuclease ABC subunit UvrB	-
K6J58_RS06010 (CHBNIII1_11330)	1240562..1241530	-	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
K6J58_RS06015 (CHBNIII1_11340)	1241533..1242153	-	621	WP_105887079.1	zinc transporter binding subunit ZevA	-
K6J58_RS06020 (CHBNIII1_11350)	1242478..1242783	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J58_RS06025 (CHBNIII1_11360)	1242794..1243117	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J58_RS06030 (CHBNIII1_11370)	1243280..1244950	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J58_RS06035 (CHBNIII1_11380)	1245012..1245353	+	342	WP_005652996.1	SirB2 family protein	-
K6J58_RS06040 (CHBNIII1_11390)	1245358..1247328	+	1971	WP_005687319.1	GNAT family N-acetyltransferase	-
K6J58_RS06045 (CHBNIII1_11400)	1247325..1247498	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32146 WP_005650215.1 NZ_AP018772:1242478-1242783 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32146 NZ_AP018772:1242478-1242783 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32146 WP_005650217.1 NZ_AP018772:1242794-1243117 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32146 NZ_AP018772:1242794-1243117 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70