Detailed information of TA system TA032142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 361060..362408
• Replicon: chromosome
• Accession: NZ_AP018772
• Organism: Haemophilus influenzae strain CHBN-III-1 isolate 15H02

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J58_RS01805
• Protein ID: WP_005687924.1
• Coordinates: 361377..362408 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2R3FM14
• Locus tag: K6J58_RS01800
• Protein ID: WP_005687922.1
• Coordinates: 361060..361380 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J58_RS01760 (CHBNIII1_03420)	356488..356766	+	279	WP_005665661.1	cell division protein FtsB	-
K6J58_RS01765 (CHBNIII1_03430)	356766..357443	+	678	WP_005655267.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J58_RS01770 (CHBNIII1_03440)	357440..357916	+	477	WP_005687916.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J58_RS01775 (CHBNIII1_03450)	358218..358652	-	435	WP_005687917.1	D-aminoacyl-tRNA deacylase	-
K6J58_RS01780 (CHBNIII1_03460)	358649..359089	-	441	WP_005687918.1	FMN-binding protein MioC	-
K6J58_RS01785 (CHBNIII1_03470)	359183..359401	-	219	WP_005650820.1	cell division protein ZapB	-
K6J58_RS01790 (CHBNIII1_03480)	359563..360564	+	1002	WP_005687920.1	class II fructose-bisphosphatase	-
K6J58_RS01795 (CHBNIII1_03490)	360764..361072	+	309	WP_005661200.1	helix-turn-helix transcriptional regulator	-
K6J58_RS01800 (CHBNIII1_03500)	361060..361380	+	321	WP_005687922.1	HipA N-terminal domain-containing protein	Antitoxin
K6J58_RS01805 (CHBNIII1_03510)	361377..362408	+	1032	WP_005687924.1	HipA domain-containing protein	Toxin
K6J58_RS01810 (CHBNIII1_03520)	362492..364150	-	1659	WP_105887059.1	thiol reductant ABC exporter subunit CydC	-
K6J58_RS01815 (CHBNIII1_03530)	364143..365888	-	1746	WP_105887060.1	ABC transporter ATP-binding protein/permease	-
K6J58_RS08840	366236..366379	+	144	WP_246589444.1	PT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39846.34 Da
• Isoelectric Point: 6.5052

>T32142 WP_005687924.1 NZ_AP018772:361377-362408 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSDYSTKGLHYLTGNKHFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDGHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQTEIHQEQLDGAMNIRDK
YGKIGADNEQYVSYEQIAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEITQEKEKVEQVYSQSFMPQPH
IDCVLKTYRKRLTLLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T32142 NZ_AP018772:361377-362408 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGACTACAGCACAAAAGGTTTACATTA
CCTTACAGGCAATAAACATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGGGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACACGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGATGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAACAAACAGAGATTCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTGCAGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTGCAGCACACTGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTGTCGGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCTTTGCCATTGTTAGCGCGAGAAGAAGGTAATGCCACACTAGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATTCCTGAAATTACACAAGAAAAGGAAAAGGTGGAGCAGGTTTATTCACAATCCTTTATGCCGCAACCGCAT
ATTGATTGCGTACTGAAAACTTATCGCAAGCGTTTAACGTTACTTAACGTATTAAATGAACCTGAGCTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12407.09 Da
• Isoelectric Point: 5.9670

>AT32142 WP_005687922.1 NZ_AP018772:361060-361380 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHRGFHFAYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
EGDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32142 NZ_AP018772:361060-361380 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCGTGGTTT
TCATTTTGCTTACAATCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGCCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCGCTCGTCCCAGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAGGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGTGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: A0A2R3FM14