Detailed information of TA system TA032130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1263617..1264256
• Replicon: chromosome
• Accession: NZ_AP018770
• Organism: Haemophilus influenzae strain CHBN-II-7 isolate 13H35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J57_RS06220
• Protein ID: WP_005650215.1
• Coordinates: 1263617..1263922 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J57_RS06225
• Protein ID: WP_005650217.1
• Coordinates: 1263933..1264256 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J57_RS06200 (CHBNII7_11750)	1259047..1261086	-	2040	WP_042599673.1	excinuclease ABC subunit UvrB	-
K6J57_RS06210 (CHBNII7_11760)	1261701..1262669	-	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
K6J57_RS06215 (CHBNII7_11770)	1262672..1263292	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
K6J57_RS06220 (CHBNII7_11780)	1263617..1263922	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J57_RS06225 (CHBNII7_11790)	1263933..1264256	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J57_RS06230 (CHBNII7_11800)	1264419..1266089	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J57_RS06235 (CHBNII7_11810)	1266151..1266492	+	342	WP_005652996.1	SirB2 family protein	-
K6J57_RS06240 (CHBNII7_11820)	1266497..1268467	+	1971	WP_042599672.1	GNAT family N-acetyltransferase	-
K6J57_RS06245 (CHBNII7_11830)	1268464..1268637	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32130 WP_005650215.1 NZ_AP018770:1263617-1263922 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32130 NZ_AP018770:1263617-1263922 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32130 WP_005650217.1 NZ_AP018770:1263933-1264256 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32130 NZ_AP018770:1263933-1264256 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70