Detailed information of TA system TA032127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 375715..377063
• Replicon: chromosome
• Accession: NZ_AP018770
• Organism: Haemophilus influenzae strain CHBN-II-7 isolate 13H35

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J57_RS01850
• Protein ID: WP_014551119.1
• Coordinates: 376032..377063 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2S9R6P7
• Locus tag: K6J57_RS01845
• Protein ID: WP_005650823.1
• Coordinates: 375715..376035 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J57_RS01805 (CHBNII7_03380)	371142..371420	+	279	WP_042593548.1	cell division protein FtsB	-
K6J57_RS01810 (CHBNII7_03390)	371420..372097	+	678	WP_042593549.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J57_RS01815 (CHBNII7_03400)	372094..372570	+	477	WP_005687916.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J57_RS01820 (CHBNII7_03410)	372875..373309	-	435	WP_013526377.1	D-aminoacyl-tRNA deacylase	-
K6J57_RS01825 (CHBNII7_03420)	373306..373746	-	441	WP_005694621.1	FMN-binding protein MioC	-
K6J57_RS01830 (CHBNII7_03430)	373843..374061	-	219	WP_005650820.1	cell division protein ZapB	-
K6J57_RS01835 (CHBNII7_03440)	374223..375224	+	1002	WP_005690169.1	class II fructose-bisphosphatase	-
K6J57_RS01840 (CHBNII7_03450)	375419..375727	+	309	WP_005661200.1	helix-turn-helix transcriptional regulator	-
K6J57_RS01845 (CHBNII7_03460)	375715..376035	+	321	WP_005650823.1	HipA N-terminal domain-containing protein	Antitoxin
K6J57_RS01850 (CHBNII7_03470)	376032..377063	+	1032	WP_014551119.1	HipA domain-containing protein	Toxin
K6J57_RS01855 (CHBNII7_03480)	377147..378805	-	1659	WP_042599482.1	thiol reductant ABC exporter subunit CydC	-
K6J57_RS01860 (CHBNII7_03490)	378798..380543	-	1746	WP_042611888.1	ABC transporter ATP-binding protein/permease	-
K6J57_RS09425	380945..381021	+	77	Protein_355	PT domain-containing protein	-
K6J57_RS09445	381090..381476	+	387	Protein_356	TonB-dependent receptor plug domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39832.44 Da
• Isoelectric Point: 6.5782

>T32127 WP_014551119.1 NZ_AP018770:376032-377063 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSGYSTKGLHYLTGNKNFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDEHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQTEIHQEQLDGAMNIRDK
YGKIGADNEQYVSYERVAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEIIQEKEKVEQIYSLSFMPQPH
IDCVLKTYRKRLALLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T32127 NZ_AP018770:376032-377063 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGGTTACAGCACAAAAGGTTTACATTA
TCTTACAGGCAATAAAAATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGAGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACACGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGACGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAACAAACAGAGATTCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTGCAGATAATGAGCAATATGTAAGTTATGAGCGTGTTGCTAAGTTTATTTTGCAGCACACAGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGTA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTGTCGGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCCTTGCCATTGTTAGCGCGAGAAGAAGGTAATGCCACACTAGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATTCCTGAAATTATACAAGAAAAGGAAAAAGTAGAGCAGATTTATTCACTATCCTTTATGCCGCAGCCACAT
ATTGATTGCGTGCTGAAAACTTATCGCAAGCGTTTAGCTTTGCTTAACGTATTAAATGAACCTGAACTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12404.04 Da
• Isoelectric Point: 5.8850

>AT32127 WP_005650823.1 NZ_AP018770:375715-376035 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHHGFHFSYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
EGDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32127 NZ_AP018770:375715-376035 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCATGGTTT
TCATTTTTCTTACAACCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGTCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCCCTCGTACCCGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAGGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGCGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: A0A2S9R6P7