Detailed information of TA system TA032124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1469858..1470497
• Replicon: chromosome
• Accession: NZ_AP018769
• Organism: Haemophilus influenzae strain CHBN-II-6 isolate 12H01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J61_RS07280
• Protein ID: WP_005650215.1
• Coordinates: 1470192..1470497 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J61_RS07275
• Protein ID: WP_005650217.1
• Coordinates: 1469858..1470181 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J61_RS07255 (CHBNII6_13840)	1465477..1465650	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
K6J61_RS07260 (CHBNII6_13850)	1465647..1467617	-	1971	WP_011961904.1	GNAT family N-acetyltransferase	-
K6J61_RS07265 (CHBNII6_13860)	1467622..1467963	-	342	WP_005652996.1	SirB2 family protein	-
K6J61_RS07270 (CHBNII6_13870)	1468025..1469695	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J61_RS07275 (CHBNII6_13880)	1469858..1470181	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J61_RS07280 (CHBNII6_13890)	1470192..1470497	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J61_RS07285 (CHBNII6_13900)	1470822..1471442	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
K6J61_RS07290 (CHBNII6_13910)	1471445..1472413	+	969	WP_048942331.1	zinc transporter permease subunit ZevB	-
K6J61_RS07300 (CHBNII6_13920)	1473028..1475067	+	2040	WP_005628806.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32124 WP_005650215.1 NZ_AP018769:c1470497-1470192 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32124 NZ_AP018769:c1470497-1470192 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32124 WP_005650217.1 NZ_AP018769:c1470181-1469858 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32124 NZ_AP018769:c1470181-1469858 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70