Detailed information of TA system TA032120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 367236..368584
• Replicon: chromosome
• Accession: NZ_AP018769
• Organism: Haemophilus influenzae strain CHBN-II-6 isolate 12H01

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J61_RS01805
• Protein ID: WP_048942801.1
• Coordinates: 367553..368584 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: Q4QMQ1
• Locus tag: K6J61_RS01800
• Protein ID: WP_011272158.1
• Coordinates: 367236..367556 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J61_RS01760 (CHBNII6_03290)	362654..362932	+	279	WP_105893802.1	cell division protein FtsB	-
K6J61_RS01765 (CHBNII6_03300)	362932..363609	+	678	WP_048942807.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J61_RS01770 (CHBNII6_03310)	363606..364082	+	477	WP_048942806.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J61_RS01775 (CHBNII6_03320)	364385..364819	-	435	WP_048942805.1	D-aminoacyl-tRNA deacylase	-
K6J61_RS01780 (CHBNII6_03330)	364816..365256	-	441	WP_048942804.1	FMN-binding protein MioC	-
K6J61_RS01785 (CHBNII6_03340)	365354..365572	-	219	WP_005650820.1	cell division protein ZapB	-
K6J61_RS01790 (CHBNII6_03350)	365734..366735	+	1002	WP_048942803.1	class II fructose-bisphosphatase	-
K6J61_RS01795 (CHBNII6_03360)	366940..367248	+	309	WP_048942802.1	helix-turn-helix transcriptional regulator	-
K6J61_RS01800 (CHBNII6_03370)	367236..367556	+	321	WP_011272158.1	HipA N-terminal domain-containing protein	Antitoxin
K6J61_RS01805 (CHBNII6_03380)	367553..368584	+	1032	WP_048942801.1	HipA domain-containing protein	Toxin
K6J61_RS01810 (CHBNII6_03390)	368668..370326	-	1659	WP_048942800.1	ABC transporter ATP-binding protein	-
K6J61_RS01815 (CHBNII6_03400)	370319..372064	-	1746	WP_110459427.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39826.37 Da
• Isoelectric Point: 6.6934

>T32120 WP_048942801.1 NZ_AP018769:367553-368584 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSGYSTKGLHYLTGNKHFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDEHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMHIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQKPMHQEQLDGAMNIRDK
YGKIGADNEQYVSYEQIAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
ETFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEITQEKEKVEQIYSQSFMPQPH
IDCVLKTYRKRLALLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T32120 NZ_AP018769:367553-368584 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGGTTACAGCACAAAAGGTTTACATTA
TCTTACAGGCAATAAACATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGAGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACATGCCACCATGCACATTATGAA
AGAATTAGGGTTTGACGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAGCAAAAACCAATGCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTGCAGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTGCAGCACACTGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCGCCAATTTATGATTTTGTGTCGGTGTCGCCCTACCCT
GAAACATTTAATTCAACACTACTTGCCTTGCCATTGTTAGCGAGAGAAGAGGGTAATGCCACACTCGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATACCTGAAATTACACAAGAAAAGGAAAAGGTGGAGCAGATTTATTCACAATCCTTTATGCCACAACCGCAT
ATTGATTGCGTACTGAAAACTTATCGCAAGCGTTTAGCGTTACTTAACGTATTAAATGAACCTGAACTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12437.12 Da
• Isoelectric Point: 5.9670

>AT32120 WP_011272158.1 NZ_AP018769:367236-367556 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHRGFHFAYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
ESDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32120 NZ_AP018769:367236-367556 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCGTGGTTT
TCATTTTGCTTACAATCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGTCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCCCTCGTCCCAGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAAGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGCGCAGTGCAAATTCAGGAAGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: Q4QMQ1