Detailed information of TA system TA032102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1504966..1505605
• Replicon: chromosome
• Accession: NZ_AP018766
• Organism: Haemophilus influenzae strain CHBN-II-3 isolate 13H41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K6J54_RS07510
• Protein ID: WP_005650215.1
• Coordinates: 1505300..1505605 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J54_RS07505
• Protein ID: WP_005650217.1
• Coordinates: 1504966..1505289 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J54_RS07485 (CHBNII3_14260)	1500585..1500758	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
K6J54_RS07490 (CHBNII3_14270)	1500755..1502725	-	1971	WP_011961904.1	GNAT family N-acetyltransferase	-
K6J54_RS07495 (CHBNII3_14280)	1502730..1503071	-	342	WP_005652996.1	SirB2 family protein	-
K6J54_RS07500 (CHBNII3_14290)	1503133..1504803	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J54_RS07505 (CHBNII3_14300)	1504966..1505289	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J54_RS07510 (CHBNII3_14310)	1505300..1505605	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J54_RS07515 (CHBNII3_14320)	1505930..1506550	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
K6J54_RS07520 (CHBNII3_14330)	1506553..1507521	+	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
K6J54_RS07530 (CHBNII3_14340)	1508136..1510175	+	2040	WP_038441065.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T32102 WP_005650215.1 NZ_AP018766:c1505605-1505300 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32102 NZ_AP018766:c1505605-1505300 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32102 WP_005650217.1 NZ_AP018766:c1505289-1504966 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32102 NZ_AP018766:c1505289-1504966 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70