Detailed information of TA system TA032100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 378872..380220
• Replicon: chromosome
• Accession: NZ_AP018766
• Organism: Haemophilus influenzae strain CHBN-II-3 isolate 13H41

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: K6J54_RS01865
• Protein ID: WP_221265029.1
• Coordinates: 379189..380220 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2S9R6P7
• Locus tag: K6J54_RS01860
• Protein ID: WP_005650823.1
• Coordinates: 378872..379192 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J54_RS01820 (CHBNII3_03430)	374293..374571	+	279	WP_042593548.1	cell division protein FtsB	-
K6J54_RS01825 (CHBNII3_03440)	374571..375248	+	678	WP_042593549.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
K6J54_RS01830 (CHBNII3_03450)	375245..375721	+	477	WP_005687916.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
K6J54_RS01835 (CHBNII3_03460)	376026..376460	-	435	WP_005690172.1	D-aminoacyl-tRNA deacylase	-
K6J54_RS01840 (CHBNII3_03470)	376457..376897	-	441	WP_005667635.1	FMN-binding protein MioC	-
K6J54_RS01845 (CHBNII3_03480)	376995..377213	-	219	WP_005650820.1	cell division protein ZapB	-
K6J54_RS01850 (CHBNII3_03490)	377375..378376	+	1002	WP_105886407.1	class II fructose-bisphosphatase	-
K6J54_RS01855 (CHBNII3_03500)	378576..378884	+	309	WP_005661200.1	helix-turn-helix transcriptional regulator	-
K6J54_RS01860 (CHBNII3_03510)	378872..379192	+	321	WP_005650823.1	HipA N-terminal domain-containing protein	Antitoxin
K6J54_RS01865 (CHBNII3_03520)	379189..380220	+	1032	WP_221265029.1	HipA domain-containing protein	Toxin
K6J54_RS01870 (CHBNII3_03530)	380304..381962	-	1659	WP_221265030.1	ABC transporter ATP-binding protein	-
K6J54_RS01875 (CHBNII3_03540)	381955..383700	-	1746	WP_221265031.1	ABC transporter ATP-binding protein/permease	-
K6J54_RS01880	383826..383972	-	147	WP_246574458.1	ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39818.41 Da
• Isoelectric Point: 6.4338

>T32100 WP_221265029.1 NZ_AP018766:379189-380220 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSGYSTKGLHYLTGNKNFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDEHFDSVNQQ
GNYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNHKEFAFVIKRFDRDEQQTEIHQEQLDGAMNIRDK
YGKIGADNEQYVSYEQIAKFILQHTENHLAQQREIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALPLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEIIQEKEKVEQIYSLSFMPQPH
IDCVLKTYRKRLALLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T32100 NZ_AP018766:379189-380220 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTAAAGAAAAATGAAGTTCATTCTGGTTACAGTACAAAAGGTTTACATTA
TCTTACAGGCAATAAAAATTTTAATCCTGAATTACCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGAATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGAGCACTTTGACAGCGTAAATCAACAG
GGAAATTATATTTTAAAGCCCTCTCCAGAAGAGTATCCTTTTTTAGCTGAAAATGAACATGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGACGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCATAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAACAAACAGAGATTCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TATGGAAAAATAGGTGCAGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTGCAGCACACTGAAAA
TCATTTAGCTCAGCAACGAGAAATTTTTCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAACAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTGTCAGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCCTTGCCATTGTTAGCGCGAGAAGAAGGTAATGCCACACTAGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATTCCTGAAATTATACAAGAAAAGGAAAAAGTAGAGCAGATTTATTCACTATCCTTTATGCCGCAGCCACAT
ATTGATTGCGTGCTGAAAACTTATCGCAAGCGTTTAGCTTTGCTTAACGTATTAAATGAACCTGAACTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12404.04 Da
• Isoelectric Point: 5.8850

>AT32100 WP_005650823.1 NZ_AP018766:378872-379192 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHHGFHFSYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
EGDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT32100 NZ_AP018766:378872-379192 [Haemophilus influenzae]
ATGCGCGATTTAGTTCGCTCGGGAAAAGTATTTCTCTACGGTGAATTTATTGGCTTACTTAGAGAAGATCATCATGGTTT
TCATTTTTCTTACAACCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGTCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCCCTCGTACCCGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAGGTGATATGTTTCGCTTTTTACTTAATAATGGTGAAAATATGTTAGGCGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: A0A2S9R6P7