Detailed information of TA system TA032094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 749878..750547
• Replicon: chromosome
• Accession: NZ_AP018765
• Organism: Haemophilus influenzae strain CHBN-II-2 isolate 12H49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R3G0F4
• Locus tag: K6J63_RS03930
• Protein ID: WP_015701504.1
• Coordinates: 749878..750261 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A502K5Z3
• Locus tag: K6J63_RS03935
• Protein ID: WP_005651224.1
• Coordinates: 750245..750547 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J63_RS03895 (CHBNII2_07490)	746638..746961	+	324	WP_048950251.1	DUF2190 family protein	-
K6J63_RS03900 (CHBNII2_07500)	746942..747253	+	312	WP_015701509.1	hypothetical protein	-
K6J63_RS03905 (CHBNII2_07510)	747256..747786	+	531	WP_042594859.1	phage tail protein	-
K6J63_RS03910 (CHBNII2_07520)	747834..748205	+	372	WP_230304754.1	phage minor tail U family protein	-
K6J63_RS03915 (CHBNII2_07530)	748208..748858	+	651	WP_070828282.1	hypothetical protein	-
K6J63_RS03920 (CHBNII2_07550)	749133..749540	+	408	WP_038440705.1	hypothetical protein	-
K6J63_RS03925 (CHBNII2_07560)	749585..749809	+	225	WP_110418021.1	DUF4035 domain-containing protein	-
K6J63_RS03930 (CHBNII2_07570)	749878..750261	+	384	WP_015701504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J63_RS03935 (CHBNII2_07580)	750245..750547	+	303	WP_005651224.1	XRE family transcriptional regulator	Antitoxin
K6J63_RS03940 (CHBNII2_07590)	750563..750799	+	237	WP_101494098.1	hypothetical protein	-
K6J63_RS03945 (CHBNII2_07600)	750851..754243	+	3393	WP_110418020.1	tape measure protein	-
K6J63_RS03950 (CHBNII2_07610)	754252..754587	+	336	WP_075913238.1	phage tail protein	-
K6J63_RS03955 (CHBNII2_07620)	754640..755290	+	651	WP_110418019.1	phage minor tail protein L	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	715186..764577	49391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14868.05 Da
• Isoelectric Point: 4.4987

>T32094 WP_015701504.1 NZ_AP018765:749878-750261 [Haemophilus influenzae]
MKQEWEVILQDPLLNWLETLAEDDVLKIYAALELLSTEGPQLSRPYADTLQGSKYTNLKELRVQSKLSVFRLFYIFDPVR
QAIVLCGGDKKGKKEKLFYKEMIALAEQTYDDYLSELTKEQENEREI

Nucleotide

• Length: 384 bp

>T32094 NZ_AP018765:749878-750261 [Haemophilus influenzae]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.21 Da
• Isoelectric Point: 9.5655

>AT32094 WP_005651224.1 NZ_AP018765:750245-750547 [Haemophilus influenzae]
MSVKFKDLMNNLPAEKQAKVKAMADDMRMELQLYRIREELELSQKQMAEALSISQPSVVALEKRGNDIKLSSVKRYIEAM
GGVLNLSVELPTGKTVTFNL

Nucleotide

• Length: 303 bp

>AT32094 NZ_AP018765:750245-750547 [Haemophilus influenzae]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAAGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Structures

Toxin

• AlphaFold DB: A0A2R3G0F4

Antitoxin

• AlphaFold DB: A0A502K5Z3