Detailed information of TA system TA032090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1640062..1640701
• Replicon: chromosome
• Accession: NZ_AP018764
• Organism: Haemophilus influenzae strain CHBN-II-1 isolate 13H34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B2WM63
• Locus tag: K6J65_RS08415
• Protein ID: WP_005694311.1
• Coordinates: 1640396..1640701 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6J65_RS08410
• Protein ID: WP_005650217.1
• Coordinates: 1640062..1640385 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J65_RS08390 (CHBNII1_16150)	1635681..1635854	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
K6J65_RS08395 (CHBNII1_16160)	1635851..1637821	-	1971	WP_011961904.1	GNAT family N-acetyltransferase	-
K6J65_RS08400 (CHBNII1_16170)	1637826..1638167	-	342	WP_005652996.1	SirB2 family protein	-
K6J65_RS08405 (CHBNII1_16180)	1638229..1639899	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
K6J65_RS08410 (CHBNII1_16190)	1640062..1640385	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K6J65_RS08415 (CHBNII1_16200)	1640396..1640701	-	306	WP_005694311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J65_RS08420 (CHBNII1_16210)	1641026..1641646	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
K6J65_RS08425 (CHBNII1_16220)	1641649..1642617	+	969	WP_038441067.1	zinc transporter permease subunit ZevB	-
K6J65_RS08435 (CHBNII1_16230)	1643233..1645272	+	2040	WP_038441065.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12248.89 Da
• Isoelectric Point: 8.0244

>T32090 WP_005694311.1 NZ_AP018764:c1640701-1640396 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYCL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T32090 NZ_AP018764:c1640701-1640396 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATTGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT32090 WP_005650217.1 NZ_AP018764:c1640385-1640062 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT32090 NZ_AP018764:c1640385-1640062 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A8B2WM63