Detailed information of TA system TA032087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 749872..750541
• Replicon: chromosome
• Accession: NZ_AP018764
• Organism: Haemophilus influenzae strain CHBN-II-1 isolate 13H34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R3G0F4
• Locus tag: K6J65_RS03925
• Protein ID: WP_015701504.1
• Coordinates: 749872..750255 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A502K5Z3
• Locus tag: K6J65_RS03930
• Protein ID: WP_005651224.1
• Coordinates: 750239..750541 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J65_RS03890 (CHBNII1_07490)	746632..746955	+	324	WP_048950251.1	DUF2190 family protein	-
K6J65_RS03895 (CHBNII1_07500)	746936..747247	+	312	WP_015701509.1	hypothetical protein	-
K6J65_RS03900 (CHBNII1_07510)	747250..747780	+	531	WP_042594859.1	phage tail protein	-
K6J65_RS03905 (CHBNII1_07520)	747828..748199	+	372	WP_230304754.1	phage minor tail U family protein	-
K6J65_RS03910 (CHBNII1_07530)	748202..748852	+	651	WP_070828282.1	hypothetical protein	-
K6J65_RS03915 (CHBNII1_07550)	749127..749534	+	408	WP_038440705.1	hypothetical protein	-
K6J65_RS03920 (CHBNII1_07560)	749579..749803	+	225	WP_110418021.1	DUF4035 domain-containing protein	-
K6J65_RS03925 (CHBNII1_07570)	749872..750255	+	384	WP_015701504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J65_RS03930 (CHBNII1_07580)	750239..750541	+	303	WP_005651224.1	XRE family transcriptional regulator	Antitoxin
K6J65_RS03935 (CHBNII1_07590)	750557..750793	+	237	WP_101494098.1	hypothetical protein	-
K6J65_RS03940 (CHBNII1_07600)	750845..754237	+	3393	WP_110418020.1	tape measure protein	-
K6J65_RS03945 (CHBNII1_07610)	754246..754581	+	336	WP_075913238.1	phage tail protein	-
K6J65_RS03950 (CHBNII1_07620)	754634..755284	+	651	WP_110418019.1	phage minor tail protein L	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	710470..764571	54101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14868.05 Da
• Isoelectric Point: 4.4987

>T32087 WP_015701504.1 NZ_AP018764:749872-750255 [Haemophilus influenzae]
MKQEWEVILQDPLLNWLETLAEDDVLKIYAALELLSTEGPQLSRPYADTLQGSKYTNLKELRVQSKLSVFRLFYIFDPVR
QAIVLCGGDKKGKKEKLFYKEMIALAEQTYDDYLSELTKEQENEREI

Nucleotide

• Length: 384 bp

>T32087 NZ_AP018764:749872-750255 [Haemophilus influenzae]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.21 Da
• Isoelectric Point: 9.5655

>AT32087 WP_005651224.1 NZ_AP018764:750239-750541 [Haemophilus influenzae]
MSVKFKDLMNNLPAEKQAKVKAMADDMRMELQLYRIREELELSQKQMAEALSISQPSVVALEKRGNDIKLSSVKRYIEAM
GGVLNLSVELPTGKTVTFNL

Nucleotide

• Length: 303 bp

>AT32087 NZ_AP018764:750239-750541 [Haemophilus influenzae]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAAGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Structures

Toxin

• AlphaFold DB: A0A2R3G0F4

Antitoxin

• AlphaFold DB: A0A502K5Z3