Detailed information of TA system TA032070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2051516..2052174
• Replicon: chromosome
• Accession: NZ_AP018756
• Organism: Metakosakonia sp. MRY16-398

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MRY16398_RS09800
• Protein ID: WP_125124251.1
• Coordinates: 2051516..2051869 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A348DBJ5
• Locus tag: MRY16398_RS09805
• Protein ID: WP_071196363.1
• Coordinates: 2051872..2052174 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRY16398_RS09785	2047970..2049424	-	1455	WP_041851859.1	AMP nucleosidase	-
MRY16398_RS09790	2049618..2050595	-	978	WP_125124250.1	oxidoreductase	-
MRY16398_RS09795	2050653..2051297	-	645	WP_041851857.1	TetR family transcriptional regulator C-terminal domain-containing protein	-
MRY16398_RS09800	2051516..2051869	+	354	WP_125124251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MRY16398_RS09805	2051872..2052174	+	303	WP_071196363.1	XRE family transcriptional regulator	Antitoxin
MRY16398_RS09810	2052251..2053201	+	951	WP_041851855.1	L,D-transpeptidase	-
MRY16398_RS09815	2053294..2053482	-	189	WP_041851854.1	YjfB family protein	-
MRY16398_RS09825	2054080..2054997	+	918	WP_041851853.1	nitrogen assimilation transcriptional regulator NAC	-
MRY16398_RS09830	2055099..2056049	+	951	WP_041851852.1	HTH-type transcriptional regulator Cbl	-
MRY16398_RS09835	2056101..2056577	-	477	WP_071196365.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13585.60 Da
• Isoelectric Point: 9.4816

>T32070 WP_125124251.1 NZ_AP018756:2051516-2051869 [Metakosakonia sp. MRY16-398]
VWEIKTTDRFDEWLIAQNDHTRASVLAALIVLRTKGPELPRPWADTIKGARYSNMKELRIQCRGEPIRPFFAFDPRRAAI
VLCAGSKAGNEKRFYAEMISRAEQEYANYLKKPEKEE

Nucleotide

• Length: 354 bp

>T32070 NZ_AP018756:2051516-2051869 [Metakosakonia sp. MRY16-398]
GTGTGGGAAATTAAAACAACGGACAGGTTTGATGAGTGGTTAATCGCTCAAAATGATCACACGCGTGCGAGCGTTTTGGC
GGCGCTGATCGTGTTGCGAACGAAAGGCCCCGAATTGCCCAGACCCTGGGCCGACACGATAAAAGGGGCGCGCTACAGCA
ATATGAAGGAGCTGCGAATTCAGTGTCGTGGGGAGCCGATACGCCCTTTTTTCGCTTTTGATCCCAGACGGGCGGCTATT
GTGTTGTGCGCAGGAAGTAAAGCTGGAAATGAAAAACGTTTCTACGCCGAAATGATTTCGCGAGCTGAGCAGGAATATGC
AAACTATCTGAAAAAACCAGAGAAAGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11067.80 Da
• Isoelectric Point: 6.2312

>AT32070 WP_071196363.1 NZ_AP018756:2051872-2052174 [Metakosakonia sp. MRY16-398]
MGRTLEQLLADEKPEVVAQAESMATETLLNIHLAELREKVQKTQVEMAHALGIRQPTVAGMEKPGRDVKLSTLKRYVEAT
GGKLRLDIELPDGSHYAFVV

Nucleotide

• Length: 303 bp

>AT32070 NZ_AP018756:2051872-2052174 [Metakosakonia sp. MRY16-398]
ATGGGCAGAACGCTGGAACAACTGCTTGCGGATGAAAAACCAGAGGTTGTGGCGCAGGCTGAGTCAATGGCGACTGAGAC
TCTCCTGAACATTCACCTGGCCGAACTGCGTGAGAAAGTGCAAAAAACGCAGGTGGAAATGGCGCATGCACTGGGGATAC
GGCAACCTACGGTGGCGGGAATGGAAAAACCGGGTCGCGATGTAAAACTGTCGACGCTTAAGCGCTACGTTGAAGCCACT
GGCGGTAAGCTTCGTCTTGATATCGAACTGCCTGATGGGTCGCATTACGCCTTCGTTGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A348DBJ5