Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3696805..3697402 Replicon chromosome
Accession NZ_AP018750
Organism Klebsiella pneumoniae strain KP64

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag D5082_RS18595 Protein ID WP_004142563.1
Coordinates 3697085..3697402 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag D5082_RS18590 Protein ID WP_004142561.1
Coordinates 3696805..3697092 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5082_RS18560 3692885..3693133 + 249 WP_002893037.1 DUF1158 domain-containing protein -
D5082_RS18565 3693151..3693492 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
D5082_RS18570 3693523..3694638 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
D5082_RS18575 3694818..3695399 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
D5082_RS18580 3695399..3695767 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
D5082_RS18585 3695887..3696537 + 651 Protein_3557 oxygen-insensitive NAD(P)H nitroreductase -
D5082_RS18590 3696805..3697092 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
D5082_RS18595 3697085..3697402 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D5082_RS18600 3697587..3698630 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
D5082_RS18610 3699297..3700163 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
D5082_RS18615 3700272..3701699 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T32039 WP_004142563.1 NZ_AP018750:c3697402-3697085 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T32039 NZ_AP018750:c3697402-3697085 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT32039 WP_004142561.1 NZ_AP018750:c3697092-3696805 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT32039 NZ_AP018750:c3697092-3696805 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References