Detailed information of TA system TA032006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1379781..1380421
• Replicon: chromosome
• Accession: NZ_AP018738
• Organism: Ferriphaselus amnicola strain OYT1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2Z6GBN9
• Locus tag: OYT1_RS06860
• Protein ID: WP_062627174.1
• Coordinates: 1379781..1380062 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OYT1_RS06865
• Protein ID: WP_197714130.1
• Coordinates: 1380104..1380421 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OYT1_RS06840	1375574..1376947	+	1374	WP_088178224.1	hypothetical protein	-
OYT1_RS06850	1377156..1378202	+	1047	WP_062627172.1	site-specific integrase	-
OYT1_RS06855	1378449..1379669	+	1221	WP_062627173.1	integrase family protein	-
OYT1_RS06860	1379781..1380062	+	282	WP_062627174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OYT1_RS06865	1380104..1380421	+	318	WP_197714130.1	HigA family addiction module antidote protein	Antitoxin
OYT1_RS06870	1380423..1380737	+	315	WP_172588573.1	type II toxin-antitoxin system MqsA family antitoxin	-
OYT1_RS06875	1380749..1381369	+	621	WP_172588519.1	hypothetical protein	-
OYT1_RS06880	1381495..1381689	+	195	WP_062627178.1	helix-turn-helix domain-containing protein	-
OYT1_RS06885	1381698..1383350	+	1653	WP_062627179.1	replication endonuclease	-
OYT1_RS06890	1383475..1384188	-	714	WP_062627180.1	hypothetical protein	-
OYT1_RS06895	1384188..1385354	-	1167	WP_062627181.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (29-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10828.34 Da
• Isoelectric Point: 9.0487

>T32006 WP_062627174.1 NZ_AP018738:1379781-1380062 [Ferriphaselus amnicola]
MIKSFSDKATAAIFYGKFVRQLPQDIQATARRKLKLIDATSRIEELRIPPGNRLELLSGDRAGQWSIRINDQWRICFNWQ
DGHAENVEIVDYH

Nucleotide

• Length: 282 bp

>T32006 NZ_AP018738:1379781-1380062 [Ferriphaselus amnicola]
ATGATCAAGTCGTTTTCAGACAAAGCCACTGCGGCCATATTTTATGGGAAGTTCGTGCGCCAACTTCCCCAAGACATACA
AGCTACGGCAAGACGCAAACTTAAGCTAATCGATGCAACTAGCCGCATCGAAGAGTTGAGGATTCCACCCGGCAATCGTC
TTGAGTTGTTAAGTGGTGATCGAGCCGGACAATGGAGCATTCGCATCAACGATCAATGGCGAATCTGCTTTAACTGGCAA
GACGGCCACGCTGAGAACGTCGAGATCGTTGATTACCACTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11770.46 Da
• Isoelectric Point: 4.6509

>AT32006 WP_197714130.1 NZ_AP018738:1380104-1380421 [Ferriphaselus amnicola]
MTWDDVSTGESLAPIHPGEVLLQDFIEPLGLTRYKVAKFTSVPQRRIDEICAGKRAITADTAMRLGRLFGMSPQTWMNLQ
SQYDLEVAEHELGDKIDHEVMPLAA

Nucleotide

• Length: 318 bp

>AT32006 NZ_AP018738:1380104-1380421 [Ferriphaselus amnicola]
ATGACATGGGACGACGTTTCCACTGGTGAGAGCCTTGCACCCATCCATCCGGGTGAGGTATTGCTGCAAGATTTCATTGA
GCCGCTAGGACTAACCCGCTACAAGGTGGCAAAGTTCACCAGCGTCCCTCAGAGGCGCATTGATGAAATCTGCGCGGGTA
AACGTGCTATCACGGCAGATACCGCTATGCGTCTAGGCCGTTTGTTTGGCATGTCTCCACAAACATGGATGAACCTGCAA
TCGCAGTACGACCTTGAGGTCGCCGAACATGAACTCGGCGACAAGATCGATCACGAAGTGATGCCGCTGGCTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A2Z6GBN9