Detailed information of TA system TA031994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 422938..423580
• Replicon: chromosome
• Accession: NZ_AP018724
• Organism: Sulfurivermis fontis strain JG42

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: EP379_RS02225
• Protein ID: WP_127475368.1
• Coordinates: 423299..423580 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EP379_RS02220
• Protein ID: WP_127478802.1
• Coordinates: 422938..423258 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP379_RS02185	418759..419397	-	639	WP_127475354.1	2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase	-
EP379_RS02190	419490..420746	-	1257	WP_127475356.1	(Fe-S)-binding protein	-
EP379_RS02195	420787..421092	+	306	WP_127475358.1	(2Fe-2S) ferredoxin domain-containing protein	-
EP379_RS02200	421493..421930	+	438	WP_127475361.1	50S ribosomal protein L13	-
EP379_RS02205	421950..422345	+	396	WP_127475363.1	30S ribosomal protein S9	-
EP379_RS02210	422385..422732	+	348	WP_127475365.1	four helix bundle protein	-
EP379_RS02220	422938..423258	-	321	WP_127478802.1	HigA family addiction module antidote protein	Antitoxin
EP379_RS02225	423299..423580	-	282	WP_127475368.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EP379_RS02230	423971..425023	+	1053	WP_172600352.1	porin	-
EP379_RS02235	425186..425317	+	132	WP_127475374.1	ribbon-helix-helix protein, CopG family	-
EP379_RS02240	425534..425779	+	246	WP_127475376.1	DUF4160 domain-containing protein	-
EP379_RS02245	425754..426047	+	294	WP_127475378.1	DUF2442 domain-containing protein	-
EP379_RS02250	426135..426833	+	699	WP_127475381.1	transposase	-
EP379_RS02255	426918..427142	+	225	WP_127475384.1	ribbon-helix-helix protein, CopG family	-
EP379_RS02260	427111..427392	+	282	WP_197722838.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EP379_RS02265	427440..427922	+	483	WP_127475386.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-77)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11177.77 Da
• Isoelectric Point: 9.8199

>T31994 WP_127475368.1 NZ_AP018724:c423580-423299 [Sulfurivermis fontis]
MITSFRDKETAALFMGERVKRYPPDIRQPARRKLAQLEQVCRVDELRIPPGNRFEALKGDRNGRWSIRINEQWRICFRFE
NGNAWDVEIVDYH

Nucleotide

• Length: 282 bp

>T31994 NZ_AP018724:c423580-423299 [Sulfurivermis fontis]
ATGATCACGTCCTTCCGCGACAAGGAAACGGCAGCCTTGTTTATGGGTGAGCGCGTGAAGCGCTATCCCCCTGATATCAG
GCAACCGGCCCGCCGCAAGCTGGCGCAATTGGAACAGGTATGTCGTGTTGATGAGTTACGTATCCCGCCGGGTAACCGTT
TCGAGGCATTGAAGGGAGATCGGAACGGCCGCTGGAGCATACGCATCAACGAGCAGTGGCGTATATGTTTTCGCTTTGAA
AACGGCAACGCCTGGGATGTGGAGATAGTGGATTACCATTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11449.04 Da
• Isoelectric Point: 5.1100

>AT31994 WP_127478802.1 NZ_AP018724:c423258-422938 [Sulfurivermis fontis]
MDFSDLVEAGDKLPAVHPGEILLHEFMTPLGLSANALAGALHVPANRISGIVNGSRSVTADTALRLARYFGTTAEFWMGL
QKDYELENARRVELAAIEKEVSPRAA

Nucleotide

• Length: 321 bp

>AT31994 NZ_AP018724:c423258-422938 [Sulfurivermis fontis]
ATGGACTTTTCCGATCTGGTTGAAGCGGGGGACAAACTGCCCGCAGTTCACCCAGGGGAGATTCTGCTGCATGAATTCAT
GACGCCGCTGGGGCTTTCCGCCAATGCCCTGGCTGGTGCGCTGCATGTACCCGCCAACCGGATCAGCGGCATCGTTAACG
GATCGCGCTCAGTGACCGCGGACACGGCACTGCGCCTGGCGCGCTATTTCGGCACTACCGCCGAATTCTGGATGGGATTG
CAAAAGGATTACGAATTGGAAAACGCCCGGCGGGTGGAGCTTGCCGCTATCGAAAAGGAAGTTTCTCCCCGCGCAGCTTG
A