Detailed information of TA system TA031993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 133798..134383
• Replicon: chromosome
• Accession: NZ_AP018724
• Organism: Sulfurivermis fontis strain JG42

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: EP379_RS00690
• Protein ID: WP_127474743.1
• Coordinates: 133798..134076 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EP379_RS00695
• Protein ID: WP_197722885.1
• Coordinates: 134099..134383 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP379_RS16450	128993..129937	-	945	WP_197722884.1	methyl-accepting chemotaxis protein	-
EP379_RS00680	131263..131877	-	615	WP_127474739.1	SPOR domain-containing protein	-
EP379_RS00685	131881..133641	-	1761	WP_127474741.1	arginine--tRNA ligase	-
EP379_RS00690	133798..134076	+	279	WP_127474743.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EP379_RS00695	134099..134383	+	285	WP_197722885.1	HigA family addiction module antidote protein	Antitoxin
EP379_RS00700	134613..134807	-	195	WP_127474747.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EP379_RS00705	134949..137138	-	2190	WP_127474749.1	primosomal protein N'	-
EP379_RS00710	137533..138924	-	1392	WP_127478780.1	HDOD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	68652..316285	247633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10649.04 Da
• Isoelectric Point: 8.2659

>T31993 WP_127474743.1 NZ_AP018724:133798-134076 [Sulfurivermis fontis]
MIKSFRCKDTQALYEGGSPRRFRAWQAQAERKLQMLDSAAVLDDLKSPPGNRLEKLSGGRAGQYSIRINDQWRVCFRWQD
GDVCDVEIVDYH

Nucleotide

• Length: 279 bp

>T31993 NZ_AP018724:133798-134076 [Sulfurivermis fontis]
ATGATCAAGTCGTTCCGCTGCAAGGATACGCAGGCGCTGTACGAGGGAGGGAGCCCGCGGCGCTTTCGCGCCTGGCAGGC
GCAGGCCGAACGCAAGCTGCAAATGCTGGATAGTGCCGCCGTCCTCGACGACCTGAAGAGTCCACCCGGCAACCGGCTGG
AAAAGCTGTCTGGCGGCCGTGCCGGGCAATACAGCATCCGCATCAACGACCAGTGGCGGGTGTGTTTCCGCTGGCAGGAC
GGCGATGTTTGCGACGTGGAAATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10565.12 Da
• Isoelectric Point: 7.3186

>AT31993 WP_197722885.1 NZ_AP018724:134099-134383 [Sulfurivermis fontis]
MRPIHPGEVLREEYLTPLGLSAHALAQALRVPATRIGEIVNERRAITPDTALRLARYFHTSAEFWMNLQTAYDLKVASAE
LAERIGREVEPRAA

Nucleotide

• Length: 285 bp

>AT31993 NZ_AP018724:134099-134383 [Sulfurivermis fontis]
ATGCGTCCCATCCATCCCGGCGAGGTTCTGCGCGAAGAGTATCTCACCCCCCTTGGCCTTTCGGCCCACGCCCTGGCCCA
GGCCCTGCGCGTGCCGGCGACGCGCATCGGCGAGATAGTCAACGAGCGCCGCGCCATCACGCCGGATACCGCTCTGCGTC
TGGCACGTTATTTCCACACCAGCGCCGAGTTCTGGATGAATCTGCAAACTGCCTACGACCTCAAGGTTGCCAGCGCCGAG
TTGGCCGAGCGTATCGGGCGCGAGGTGGAGCCACGCGCGGCGTAG