Detailed information of TA system TA031944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14747..15404
• Replicon: plasmid pGSU10-3-1
• Accession: NZ_AP018672
• Organism: Klebsiella pneumoniae strain GSU10-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: D6O76_RS27815
• Protein ID: WP_000270043.1
• Coordinates: 15054..15404 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6O76_RS27810
• Protein ID: WP_000124640.1
• Coordinates: 14747..15049 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6O76_RS27760	10372..10800	+	429	WP_000591074.1	hypothetical protein	-
D6O76_RS27765	10857..11216	+	360	WP_000422768.1	hypothetical protein	-
D6O76_RS27770	11216..11662	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
D6O76_RS27775	11659..12177	+	519	WP_000210756.1	nitrite reductase	-
D6O76_RS27780	12177..12407	+	231	WP_000972663.1	hypothetical protein	-
D6O76_RS27785	12394..13251	+	858	WP_001167032.1	hypothetical protein	-
D6O76_RS27795	13482..14009	+	528	WP_001236377.1	thermonuclease family protein	-
D6O76_RS27800	14067..14339	+	273	WP_001043047.1	HU family DNA-binding protein	-
D6O76_RS27805	14427..14720	+	294	WP_001239997.1	chromosome segregation protein ParM	-
D6O76_RS27810	14747..15049	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
D6O76_RS27815	15054..15404	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6O76_RS27820	15567..16115	+	549	WP_001061574.1	transcriptional regulator	-
D6O76_RS27830	16456..16650	+	195	WP_000343597.1	hypothetical protein	-
D6O76_RS27835	16661..17032	+	372	WP_000516916.1	hypothetical protein	-
D6O76_RS27840	17025..17495	+	471	WP_001281821.1	hypothetical protein	-
D6O76_RS27845	17510..17845	-	336	WP_000683476.1	hypothetical protein	-
D6O76_RS27850	17942..18430	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
D6O76_RS27855	18433..18930	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / qnrA1 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..159072	159072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T31944 WP_000270043.1 NZ_AP018672:c15404-15054 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T31944 NZ_AP018672:c15404-15054 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT31944 WP_000124640.1 NZ_AP018672:c15049-14747 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT31944 NZ_AP018672:c15049-14747 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9