Detailed information of TA system TA031832

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 5536995..5537407
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: ECpv15279_RS28370
• Protein ID: WP_000132630.1
• Coordinates: 5536995..5537336 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	5537331..5537407 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS29640	5532563..5532763	+	201	WP_001310455.1	hypothetical protein	-
ECpv15279_RS28350	5533006..5534418	-	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
ECpv15279_RS28355	5534595..5534759	+	165	WP_000394274.1	DUF1127 domain-containing protein	-
ECpv15279_RS28360	5534856..5535737	+	882	WP_001476351.1	DUF262 domain-containing protein	-
ECpv15279_RS28365	5535785..5536948	+	1164	WP_001304006.1	DUF1524 domain-containing protein	-
ECpv15279_RS28370	5536995..5537336	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	5537331..5537407	+	77	NuclAT_14	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_14	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_14	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_14	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_15	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_15	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_15	-	Antitoxin
-	5537331..5537407	+	77	NuclAT_15	-	Antitoxin
ECpv15279_RS28375	5537557..5539311	-	1755	WP_000110082.1	restriction endonuclease subunit S	-
ECpv15279_RS28380	5539311..5540780	-	1470	WP_001302468.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T31832 WP_000132630.1 NZ_AP018488:c5537336-5536995 [Escherichia coli O157:H7]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T31832 NZ_AP018488:c5537336-5536995 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT31832 NZ_AP018488:5537331-5537407 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin